Exeter/10 July 2013

From 2013.igem.org

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Latest revision as of 19:53, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Morning

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.

MiniPrep of the following Parts:

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

Plasmids extracted are now in storage at -20°C.

Afternoon

Liquid cultures of the blue light module and magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator

- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate

- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)

We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli

@@ Line 1: / Line 1: @@
-=Liquid culture=
+{{:Team:Exeter/Template/Header}}
+<div class="container">
+    <div class="row">
+        <div class="span" style="text-align:justify">
-* K592010 (Yellow)
+== Morning ==
-* K592012 (Magenta
+- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.
-* B0015
-* K592003 (green promoter)
-* Q04510 (lambda cl)
-* K592005 (FixJ)
-* K592002 (CcaR)
-* K592001 (CcaS)
-* K592004 (YF1)
-Transform B0034 again
+MiniPrep of the following Parts:
+- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment
+- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
+- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
+Plasmids extracted are now in storage at -20&deg;C.
+==Afternoon==
+'''Liquid cultures of the blue light module and magenta pigment'''
+- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
+- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
+- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator
+- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate
+- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor
+'''Retry of BBa_B0034 transformation'''
+Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
+==(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)==
+We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.
+Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
+   </div>
+</div>
+{{:Team:Exeter/Template/Footer}}