Team:EPF Lausanne/Calendar/20 August 2013

From 2013.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 3: Line 3:
''Gibson Assembly'' <br>
''Gibson Assembly'' <br>
-
<br>- We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).
+
- We did the streptavidin alive gibson assembly reaction (NEB protocol).
<br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.
<br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.
 +
<br>- Gibson Assembly probably didn't work.
<br><br>
<br><br>
Line 10: Line 11:
''Gradient PCR of Backbones'' <BR>
''Gradient PCR of Backbones'' <BR>
-
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.
+
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the right size.
<BR> <BR>
<BR> <BR>

Latest revision as of 21:56, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Assembly
- We did the streptavidin alive gibson assembly reaction (NEB protocol).
- Verification of the gibson reaction with a 0.8% gel electrophoresis.
- Gibson Assembly probably didn't work.

Sensing-Effector

Gradient PCR of Backbones
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the right size.

Nanoparicles

Making Nanoparticles, 2nd try
- Biotinylation of the new nanoparticles.
- Dialysis of biotinylated nanoparticles.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/20_August_2013"