"
Team:DTU-Denmark/Notebook/6 July 2013
From 2013.igem.org
(Difference between revisions)
(→208 lab) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:DTU-Denmark/Templates/StartPage|}} | + | {{:Team:DTU-Denmark/Templates/StartPage|6 July 2013}} |
| - | + | Navigate to the [[Team:DTU-Denmark/Notebook/5_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/7_July_2013|Next]] Entry | |
| - | + | ||
| - | + | ||
=208 lab= | =208 lab= | ||
<hr/> | <hr/> | ||
| Line 15: | Line 13: | ||
==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| - | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html | + | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though. |
Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | ||
Latest revision as of 20:39, 16 September 2013
6 July 2013
Contents |
208 lab
Main purposes today
Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
Who was in the lab
Kristian, Helen
Procedure
Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
Conclusion from today
The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
Navigate to the Previous or the Next Entry
