Team:UGent

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A new model for chromosomal evolution: Eliminating antibiotic resistance.
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<p>Welcome to the wiki page of 2013's UGent iGEM team. This summer we will attempt to <b>improve an existing technique</b>, called CIChE, that raises the yield of production of biochemical products using micro- organisms as production hosts. We will <b>eliminate</b> CIChE's need for <b>antibiotic resistance genes</b> by implementing a bacterial <b>toxin-antitoxin system</b>.</p>
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<p>We believe that with our adaptation CIChE may become a <b>very valuable technique</b>; useful in everyday industrial biotechnology and in a myriad of applications in synthetic biology. <br> <br>Explore this wiki to find out more about our project and other activities during our iGEM summer! </p>
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Ghent University has not yet participated in this world-renowned competition in synthetic biology. That, however, is about to change.
 
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''''' A new model for stabilised gene duplication: Eliminating antibiotic resistance '''''
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The main goal of the industrial biotechnology is to increase the yield of the synthesis of biochemical products using microorganisms as production hosts. In general, this includes engineering large synthetic pathways and improving their expression. Overexpression of endogenous and/or heterologous genes has hitherto mainly been achieved by using high or medium copy plasmids. However, studies have demonstrated that cells containing plasmids for the overexpression of the desired product lose their productivity fairly quickly as a result of genetic instability. To avoid these problems a new method was developed for the overexpression of a gene of interest in the bacterial chromosome: Chemically Inducible Chromosomal evolution or CIChE. In this technique the chromosome is evolved to contain a higher number of gene copies by adding a chemical inducer. The original model for CIChE, however, results in bacterial strains containing a large number of antibiotic resistance genes. To make this valuable technique more widely applicable in the industry, we developed a new model for chromosomal evolution, based on a toxin-antitoxin system instead of antibiotic resistance.
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Latest revision as of 03:00, 4 October 2013

UGent 2013 Banner.jpg


A new model for chromosomal evolution: Eliminating antibiotic resistance.



Welcome to the wiki page of 2013's UGent iGEM team. This summer we will attempt to improve an existing technique, called CIChE, that raises the yield of production of biochemical products using micro- organisms as production hosts. We will eliminate CIChE's need for antibiotic resistance genes by implementing a bacterial toxin-antitoxin system.

We believe that with our adaptation CIChE may become a very valuable technique; useful in everyday industrial biotechnology and in a myriad of applications in synthetic biology.

Explore this wiki to find out more about our project and other activities during our iGEM summer!




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We thank following sponsors for their support

Bio Base Europe Pilot Plant
Inbio
Bioké Novolab
MRP UGent
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