Exeter/7 August 2013
From 2013.igem.org
(Difference between revisions)
GeorgiaDrew (Talk | contribs) (Created page with " Glycerol stocks *200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs. *Store at -80 degrees celsius *Stocks made of: NEB1, Promotor + RBS, NEB...") |
(→We miniprepped:) |
||
(3 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
+ | {{:Team:Exeter/Template/Header}} | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="span" style="text-align:justify"> | ||
Glycerol stocks | Glycerol stocks | ||
Line 4: | Line 8: | ||
*Store at -80 degrees celsius | *Store at -80 degrees celsius | ||
*Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow | *Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow | ||
+ | |||
+ | |||
+ | ==We miniprepped:== | ||
+ | |||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM | ||
+ | |||
+ | * 3 NEB (RFP and Terminator) AMP | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM | ||
+ | |||
+ | * NEB4 (RBS and cph8) AMP | ||
+ | |||
+ | * NEB1 (RBS and cph8) AMP | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM | ||
+ | |||
+ | * [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM | ||
+ | |||
+ | == Adam and Tom == | ||
+ | |||
+ | |||
+ | Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel) | ||
+ | |||
+ | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | {{:Team:Exeter/Template/Footer}} |
Latest revision as of 19:56, 2 October 2013
Glycerol stocks
- 200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
- Store at -80 degrees celsius
- Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow
We miniprepped:
- [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP
- [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
- [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
- [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM
- [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
- 3 NEB (RFP and Terminator) AMP
- [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
- NEB4 (RBS and cph8) AMP
- NEB1 (RBS and cph8) AMP
- [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
- [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM
Adam and Tom
Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)
Take me back to the notebook.