Team:Peking

From 2013.igem.org

(Difference between revisions)
(Prototype team page)
 
(697 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<style type="text/css">
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
/* hiding the top section*/
-
This is a template page. READ THESE INSTRUCTIONS.
+
body{position:absolute; top:0px; width:100%; height:1300px;}
-
</div>
+
#top-section{
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
  height:0px;
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
    border:none;
 +
    width:980px;
 +
    margin:0 auto;
 +
    padding:0 0 0 0;
 +
    background-color:transparent;
 +
    overflow:hide;
 +
 
 +
}
 +
#p-logo{display:none;}
 +
#search-controls{display:none;}
 +
#top{display:none;}
 +
.firstHeading{display:none;}
 +
/* end of hiding the top section*/
 +
 
 +
/* global setting*/
 +
#globalWrapper{position:absolute; top:0px;left:0px; width:100%; padding:0 0 0 0;margin:0 0 0 0;height:100%;font-family: calibri， Arial, Helvetica, sans-serif;}
 +
#content{
 +
    background-color:transparent;
 +
    border:none;
 +
    width:1200px;
 +
    height:700px;
 +
    marign:0 auto;
 +
    padding:0 0 0 0;
 +
    top:-40px;
 +
    }
 +
#bodyContent{
 +
    background-color:transparent;
 +
    border:none;
 +
   
 +
    }
 +
siteSub{
 +
    display:none;
 +
    border:none;
 +
    }
 +
contentSub{
 +
    display:none;
 +
    }
 +
/* end of global setting*/
 +
 
 +
/*hiding the footer-box*/
 +
#footer-box{display:none;}
 +
#catlinks{display:none;}
 +
/*end of hiding the footer book*/
 +
 
 +
/* menu (page, edit ...) */
 +
#menubar {
 +
    background-color:transparent;
 +
    position: relative;
 +
    float:left;
 +
    white-space: nowrap;
 +
    top:-6px;
 +
    width: 490px;
 +
    z-index: 5000;
 +
    font-family: sans-serif;
 +
    font-size: 95%;
 +
    line-height: 1em;
 +
    z-index:99;
 +
}
 +
 
 +
 
 +
.left-menu, .left-menu a {
 +
    text-align: left;
 +
    color:#999999;
 +
    text-transform: lowercase;
 +
}
 +
 
 +
.left-menu:hover {
 +
    color: #D00000;
 +
    background-color: transparent;
 +
}
 +
 
 +
.right-menu{width:400px; float:right}
 +
.right-menu, .right-menu a {
 +
    right: 0px;
 +
    text-align: right;
 +
    color: #999999;
 +
}
 +
#menubar ul {
 +
    color: #999999;
 +
    list-style: none;
 +
}
 +
#menubar li {
 +
    display: inline;
 +
    position: relative;
 +
    cursor: pointer;
 +
    padding-left: 0px;
 +
    padding-right: 0px;
 +
}
 +
.left-menu li a {
 +
    padding: 0px 10px 0px 0px;
 +
}
 +
.left-menu .selected {
 +
#    color: #999999;
 +
}
 +
#.left-menu .selected:hover {
 +
#    color: #999999;
 +
#}
 +
 
 +
.left-menu:hover a {
 +
    color: #999999;;
 +
}
 +
 
 +
.left-menu li a:hover {
 +
    color: #D00000;
 +
    text-decoration: underline;
 +
}
 +
 
 +
 
 +
.right-menu li {
 +
#    background-color: transparent;
 +
}
 +
.right-menu li a {
 +
    padding: 0px 15px 0px 0px;
 +
    color: #999999;;
 +
    background-color: transparent;
 +
}
 +
.right-menu li a:hover {
 +
    color: #D00000;
 +
    text-decoration: underline;
 +
}
 +
/* end menu (edit, page ...) */
 +
 
 +
/* navigation bar*/
 +
 
 +
#navigationblock{background-color:#fafaf8; position:fixed; top:0px; width:1200px; height:130px; overflow:hidden; font-family:Calibri, Arial; float:left; margin:0px; padding:0px; z-index:1000; border-bottom:1px solid #d3d7d8;}
 +
#navbarlogo{position:absolute; top:0px; left:0px; width:1200px}
 +
#navigationbar{position:absolute; left:460px; top:38px; font-size:16px; font-weight:100; color:black;}
 +
#navigationbar > li {display:block; list-style-type:none; float:left; width:75px; height:30px; text-align:center; }
 +
 
 +
.Navbar_Item{background-color:transparent;}
 +
.Navbar_Item a {color:black; text-decoration:none;}
 +
.Navbar_Item > ul {display:none; width:900px; height:80px; text-align:center; color:black; z-index:1100; }
 +
.Navbar_Item > ul > li {float:left; list-style:none; text-align:center; background-color:transparent; position:relative; top:10px; padding:0 10px;}
 +
.Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;}
 +
.Navbar_Item:hover > ul{zoom:1; display:block;}
 +
.Navbar_Item:hover >a {color:#D00000; background-color:#fafaf8;}
 +
.Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;}
 +
.Navbar_Item > ul > li:hover >a {color:#D00000}
 +
.BackgroundofSublist{position:absolute; left:-1000px; width:2000px; height:80px; background-color:#ffffff; opacity:0;}
 +
 
 +
 
 +
#Home_Sublist{position:relative; top:0px; left:-50px;}
 +
#Team_Sublist{position:relative; top:0px; left:-110px;}
 +
#Project_Sublist{position:relative; top:0px; left:-180px;}
 +
#Model_Sublist{position:relative; top:0px; left:-140px;}
 +
#DataPage_Sublist{position:relative; top:0px; left:-60px;}
 +
#Safety_Sublist{position:relative; top:0px; left:-180px;}
 +
#HumanPractice_Sublist{position:relative; top:0px; left:-470px;}
 +
 
 +
#iGEM_logo{position:absolute; top:30px; left:1090px; height:80px;}
 +
/*end navigation bar*/
 +
 
 +
/*Major body*/
 +
#MajorBody{position:absolute; top:0px; left:0px; width:1200px; height:1380px; background-color:#f9f9f7;}
 +
#ProjectTitle{position:absolute; left:0px; width:1200px; height:184px; top:150px; z-index:600;}
 +
#TitleBackground{position:absolute; left:0px; top:0px; width:1200px;}
 +
#LittleScout{position:relative; left:0px; top:-3px; width:70px;}
 +
#ProjectName{position:absolute; left:405px; top:20px; border-bottom:0px; color:#FFFFFF; font-size:48px; font-family:calibri,Arial, Helvetica, sans-serif;}
 +
#ProjectSubname{position:absolute; left:0px; width:1200px; top:105px; border-bottom:0px; color:#FFFFFF; font-size:18px;  font-family: calibri， Arial, Helvetica, sans-serif; text-align:center;}
 +
 
 +
#MajorBodyBackground{position:absolute; top:334px; left:0px; width:1200px;}
 +
 
 +
#PreLocation{font-size:14px; font-family:arial,helvetica,sans-serif; color:#ca4321;position:absolute;top:190px;left:0px;text-align:center;width:1200px;line-height:20px; font-weight:bold;}
 +
 
 +
#AbstractBox{position:absolute; left:430px; top:390px; width:710px; height:540px; background-color:transparent;}
 +
#AbstractBackground{width:100%; height:100%; background-color:#FFFFFF; opacity:0.8;}
 +
#AbstractContent{position:absolute; left:40px; top:25px; width:630px; font-size:18px; line-height:25px ; font-family: calibri, Arial, sans-serif; text-align:justify;}
 +
#AbstractContent:first-letter{font-size:30px;}
 +
.SmallBoxes{width:340px; height:250px;background-color:#FFFFFF; overflow:hidden;font-family: calibri， Arial, Helvetica, sans-serif; }
 +
.SmallBoxes > img{position:absolute; left:0px; top:0px;}
 +
.SmallBoxes p{position:absolute; left:20px; top:75px; width:300px; font-size:14px; color:#ffffff; text-align:justify;}
 +
.SmallBoxes a{position:absolute; left:240px; top:220px; width:300px; font-size:12px; color:#ffffff; text-align:justify;}
 +
 
 +
 
 +
#AppendixBox1{position:absolute; left:60px; top:390px; background-color:#aa4630;}
 +
#Appendix1Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix1Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox2{position:absolute; left:60px; top:680px; background-color:#e98d70;}
 +
#Appendix2Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix2Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox3{position:absolute; left:60px; top:970px; background-color:#f37021;}
 +
#Appendix3Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix3Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox4{position:absolute; left:430px; top:970px; background-color:#1f8a70;}
 +
#Appendix4Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix4Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox5{position:absolute; left:800px; top:970px; background-color:#004258;}
 +
#Appendix5Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix5Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
 
 +
.AppendixHeadLine{position:absolute; left:20px; top:20px; width:250px;border-bottom:0px; font-size:23px; color:#ffffff;}
 +
.AppendixIcon{position:absolute; left:270px; top:20px; width:50px; }
 +
 
 +
 
 +
/*end of Major body*/
 +
#AcknowledgementBox{ position:absolute; left:0px; top:1280px; width:1200px;}
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
<!--navigationbar-->
 +
 
 +
<div id="navigationblock">
 +
<img id="navbarlogo" src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg"/>
 +
       
 +
<ul id="navigationbar">
 +
<li id="PKU_navbar_Home" class="Navbar_Item">
 +
                       
 +
<a href="https://2013.igem.org/Team:Peking">Home</a>
 +
<ul id="Home_Sublist" >
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_Team" class="Navbar_Item">
 +
<a href="">Team</a>
 +
<ul id="Team_Sublist">
 +
                                <div class="BackgroundofSublist"></div>
 +
<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Team/Attributions">Attributions</a></li>
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_Project" class="Navbar_Item">
 +
<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
 +
<ul id="Project_Sublist">
 +
                                <div class="BackgroundofSublist"></div>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_Model" class="Navbar_Item">
 +
<a href="">Model</a>
 +
<ul id="Model_Sublist">
 +
                                <div class="BackgroundofSublist"></div>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/Model">Band-pass Filter</a></li>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
 +
</ul>
 +
</li>
 +
                        <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px">
 +
<a href="">Data page</a>
 +
<ul id="DataPage_Sublist">
 +
                                <div class="BackgroundofSublist"></div>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
 +
 
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_Safety" class="Navbar_Item">
 +
<a href="https://2013.igem.org/Team:Peking/Safety">Safety</a>
 +
<ul id="Safety_Sublist">
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px">
 +
<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
 +
<ul id="HumanPractice_Sublist">
 +
                                <div class="BackgroundofSublist"></div>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire Survey</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
 +
 
 +
</ul>
 +
</li>
 +
 +
</ul>
 +
        <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
<!--end navigationbar-->
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
<!--MajorBody-->
 +
<div id="MajorBody"> 
 +
<div id="ProjectTitle">
 +
                <img id="TitleBackground" src="https://static.igem.org/mediawiki/igem.org/c/c5/Peking2013_home_title.jpg" />
 +
               
 +
<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
 +
                <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
 +
 +
<p id="PreLocation">
 +
<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
 +
      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
 +
</p>
-
{|align="justify"
+
</div>
-
|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
        <img id="MajorBodyBackground" src="https://static.igem.org/mediawiki/igem.org/9/97/Peking2013_Home_background.jpg" />
-
|[[Image:Peking_logo.png|200px|right|frame]]
+
        <div id="AbstractBox">
-
|-
+
            <div id="AbstractBackground"></div>
-
|
+
              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.  
-
''Tell us more about your project. Give us background.  Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
<br/><br/>
-
|[[Image:Peking_team.png|right|frame|Your team picture]]
+
Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.
-
|-
+
<br/><br/>
-
|
+
Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.  
-
|align="center"|[[Team:Peking | Team Peking]]
+
<br/><br/>
-
|}
+
We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
 +
</p>
 +
        </div>
 +
        <div id="AppendixBox1" class="SmallBoxes" >
 +
              <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
 +
              <div id="Appendix1Cover" >
 +
                    <img id="AppendixImg1" src="https://static.igem.org/mediawiki/igem.org/e/ec/Peking2013_homepage_appendix1.jpg" />
 +
                    <h1 class="AppendixHeadLine">Biosensor Mining</h1>
 +
                    <img id="Appendix1Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/1/13/Peking2013_home_appendix1icon.png" />
 +
                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
 +
              </div>
 +
        </div>
 +
        <div id="AppendixBox2" class="SmallBoxes">
 +
              <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
 +
              <div id="Appendix2Cover" >
 +
                    <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
 +
                    <h1 class="AppendixHeadLine">Biosensors</h1>
 +
                    <img id="Appendix2Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
 +
                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
 +
              </div>
 +
        </div>
 +
        <div id="AppendixBox3" class="SmallBoxes">
 +
              <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
 +
              <div id="Appendix3Cover" >
 +
                    <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
 +
                    <h1 class="AppendixHeadLine">Adaptors</h1>
 +
                    <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
 +
                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
 +
              </div>
 +
        </div>
 +
        <div id="AppendixBox4" class="SmallBoxes">
 +
              <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
 +
              <div id="Appendix4Cover" >
 +
                    <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
 +
                    <h1 class="AppendixHeadLine">Band-pass Filter</h1>
 +
                    <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
 +
                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
 +
              </div>
 +
        </div>
 +
        <div id="AppendixBox5" class="SmallBoxes">
 +
              <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
 +
              <div id="Appendix5Cover" >
 +
                    <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
 +
                    <h1 class="AppendixHeadLine">Human Practice</h1>
 +
                    <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
 +
                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
 +
              </div>
 +
        </div>       
 +
 +
</div><!--end of major body-->
 +
<img id="AcknowledgementBox" src="https://static.igem.org/mediawiki/igem.org/c/c4/Peking_Sponsors.jpg">
 +
<!--end of acknowledgement box-->
-
<!--- The Mission, Experiments --->
+
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script>
 +
<script type="text/javascript">
 +
$(window).scroll(function(){
 +
  if(0==document.getElementById("content").offsetLeft){ $('#navigationblock').css('left',-$(window).scrollLeft());}
 +
  else{$('#navigationblock').css('left',document.getElementById("content").offsetLeft); }
 +
});
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
$(window).resize(function(){
-
!align="center"|[[Team:Peking|Home]]
+
  if(0==document.getElementById("content").offsetLeft){ $('#navigationblock').css('left',-$(window).scrollLeft());}
-
!align="center"|[[Team:Peking/Team|Team]]
+
  else{$('#navigationblock').css('left',document.getElementById("content").offsetLeft); }
-
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Peking Official Team Profile]
+
});
-
!align="center"|[[Team:Peking/Project|Project]]
+
 
-
!align="center"|[[Team:Peking/Parts|Parts Submitted to the Registry]]
+
document.getElementById("AppendixBox1").onmouseover=function(){MoveInSlide("#Appendix1Cover")};
-
!align="center"|[[Team:Peking/Modeling|Modeling]]
+
document.getElementById("AppendixBox1").onmouseout=function(){MoveOutSlide("#Appendix1Cover")};
-
!align="center"|[[Team:Peking/Notebook|Notebook]]
+
document.getElementById("AppendixBox2").onmouseover=function(){MoveInSlide("#Appendix2Cover")};
-
!align="center"|[[Team:Peking/Safety|Safety]]
+
document.getElementById("AppendixBox2").onmouseout=function(){MoveOutSlide("#Appendix2Cover")};
-
!align="center"|[[Team:Peking/Attributions|Attributions]]
+
document.getElementById("AppendixBox3").onmouseover=function(){MoveInSlide("#Appendix3Cover")};
-
|}
+
document.getElementById("AppendixBox3").onmouseout=function(){MoveOutSlide("#Appendix3Cover")};
 +
document.getElementById("AppendixBox4").onmouseover=function(){MoveInSlide("#Appendix4Cover")};
 +
document.getElementById("AppendixBox4").onmouseout=function(){MoveOutSlide("#Appendix4Cover")};
 +
document.getElementById("AppendixBox5").onmouseover=function(){MoveInSlide("#Appendix5Cover")};
 +
document.getElementById("AppendixBox5").onmouseout=function(){MoveOutSlide("#Appendix5Cover")};
 +
 
 +
function MoveInSlide(SlideId)
 +
{
 +
$(SlideId).stop().animate({top:"0px"},"fast");
 +
         
 +
};
 +
 
 +
 
 +
 
 +
function MoveOutSlide(SlideId)
 +
{
 +
$(SlideId).stop().animate({top:"170px"},"fast");
 +
       
 +
};
 +
 
 +
 
 +
</script>
 +
 
 +
</html>

Latest revision as of 18:10, 28 October 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment

★Presentation: Room 34-101, Saturday 12:30 PM, Session 2     ★Poster: Sunday, #13 (Stata)

Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.

Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.

Mining aromatics-sensing Biobricks
from the genomic database

Biosensor Mining

The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot.

LEARN MORE

High-performance, profile-
specific biosensors

Biosensors

A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.

LEARN MORE

Converting the undetectable into
the detectable

Adaptors

To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.

LEARN MORE

Rapidly determining
the analyte concentration

Band-pass Filter

For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples.

LEARN MORE

Communications, Questionnaire survey,
Factory Visit and interview

Human Practice

To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.

LEARN MORE

Retrieved from "http://2013.igem.org/Team:Peking"