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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog
From 2013.igem.org
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| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
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- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. | - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. | ||
| - | - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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<font size="4"> '''8/3/13''' </font> | <font size="4"> '''8/3/13''' </font> | ||
| - | - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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- Started E coli liquid culture over night | - Started E coli liquid culture over night | ||
* 10mL of BL21 for spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | * 10mL of BL21 for spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]]. | ||
| - | * 2mL each of BL21, W3110, and B for phage viability and infection test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | * 2mL each of BL21, W3110, and B for phage viability and infection test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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<font size="4"> '''8/5/13''' </font> | <font size="4"> '''8/5/13''' </font> | ||
| - | - Performed Phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Performed Phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Discussed ideas for modeling phage plaque sizes | - Discussed ideas for modeling phage plaque sizes | ||
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<font size="4"> '''8/6/13''' </font> | <font size="4"> '''8/6/13''' </font> | ||
| - | - Check up on the phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - | + | - Check up on the phage viability/infection test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Started 5mL of E coli B liquid culture overnight. | - Started 5mL of E coli B liquid culture overnight. | ||
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<font size="4"> '''8/7/13''' </font> | <font size="4"> '''8/7/13''' </font> | ||
| - | - Performed phage | + | - Performed spot test to estimate phage titerfor [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
- Phage Purification Group started the purification process with CsCl gradient. | - Phage Purification Group started the purification process with CsCl gradient. | ||
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| - | <font size="4"> ''' | + | <font size="4"> '''8/9/13''' </font> |
| - | - | + | - Performed Preliminary Titer for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]]. |
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| + | <font size="4"> '''8/11/13''' </font> | ||
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| + | - Started approximately 20mL of E coli B liquid culture overnight. | ||
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| + | <font size="4"> '''8/12/13''' </font> | ||
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| + | - Performed titer - repeat for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]] based on the results from 8.10 preliminary titer test. | ||
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| + | - Started T1 propagation. | ||
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| + | - Made accurate x2 top agar as preparation for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]]. | ||
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| + | <br> | ||
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| + | <font size="4"> '''8/13/13''' </font> | ||
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| + | - Started approximately 20mL of E coli B liquid culture overnight. | ||
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| + | <font size="4"> '''8/14/13''' </font> | ||
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| + | - Performed mutagenesis and spot test for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.14_Mutagen_Concentration_Test_-_Seventh_Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]]. | ||
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| + | - Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions. | ||
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| + | <font size="4"> '''8/15/13''' </font> | ||
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| + | - T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 10<sup>9</sup> pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration. | ||
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| + | - Started approximately 30mL of E coli B liquid culture overnight. | ||
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| + | <br> | ||
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| + | <font size="4"> '''8/16/13''' </font> | ||
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| + | - Started [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]]. | ||
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| + | - Phage Purification team performed the CsCl gradient for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.14_Mutagen_Concentration_Test_-_Seventh_Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]]. | ||
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| + | - Started approximately 10 mL of E coli B liquid culture overnight. | ||
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Latest revision as of 15:37, 9 September 2013
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8/1/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Discussed ideas for modeling phage plaque sizes
8/6/13 - Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Started 5mL of E coli B liquid culture overnight.
8/7/13 - Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Phage Purification Group started the purification process with CsCl gradient.
8/8/13 - Started approximately 15mL of E coli B liquid culture overnight.
8/9/13 - Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/11/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/12/13 - Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test. - Started T1 propagation. - Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.
8/13/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/14/13 - Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol. - Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.
8/15/13 - T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 109 pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration. - Started approximately 30mL of E coli B liquid culture overnight.
8/16/13 - Started 8.16 Modeling Phage Plaque Sizes - Experiment One. - Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol. - Started approximately 10 mL of E coli B liquid culture overnight.
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