Team:Peking

From 2013.igem.org

(Difference between revisions)
 
(370 intermediate revisions not shown)
Line 2: Line 2:
<style type="text/css">
<style type="text/css">
/* hiding the top section*/
/* hiding the top section*/
-
body{position:absolute; top:0px; width:100%; height:1480px;}
+
body{position:absolute; top:0px; width:100%; height:1300px;}
#top-section{
#top-section{
   height:0px;
   height:0px;
Line 20: Line 20:
/* global setting*/
/* global setting*/
-
#globalWrapper{position:absolute; top:0px;left:0px; width:100%; padding:0 0 0 0;margin:0 0 0 0;height:100%;}
+
#globalWrapper{position:absolute; top:0px;left:0px; width:100%; padding:0 0 0 0;margin:0 0 0 0;height:100%;font-family: calibri， Arial, Helvetica, sans-serif;}
#content{
#content{
     background-color:transparent;
     background-color:transparent;
     border:none;
     border:none;
-
     width:1350px;
+
     width:1200px;
     height:700px;
     height:700px;
     marign:0 auto;
     marign:0 auto;
Line 129: Line 129:
/* navigation bar*/
/* navigation bar*/
-
#navigationblock{background-color:#fafaf8; position:fixed; top:0px; width:1350px; height:130px; overflow:hidden; font-family:Microsoft YaHei,Calieri, Arial; float:left; margin:0px; padding:0px; z-index:1000; border-bottom:1px solid #d3d7d8;}
+
#navigationblock{background-color:#fafaf8; position:fixed; top:0px; width:1200px; height:130px; overflow:hidden; font-family:Calibri, Arial; float:left; margin:0px; padding:0px; z-index:1000; border-bottom:1px solid #d3d7d8;}
-
#navbarlogo{position:relative; top:8px; float:left; height:120px;}
+
#navbarlogo{position:absolute; top:0px; left:0px; width:1200px}
-
#navigationbar{position:absolute; left:600px; top:28px; font-size:16px; font-weight:100; color:black;}
+
#navigationbar{position:absolute; left:460px; top:38px; font-size:16px; font-weight:100; color:black;}
-
#navigationbar > li {display:block; list-style-type:none; float:left; width:85px; height:100px; text-align:center; background-color:transparent;}
+
#navigationbar > li {display:block; list-style-type:none; float:left; width:75px; height:30px; text-align:center; }
 +
.Navbar_Item{background-color:transparent;}
.Navbar_Item a {color:black; text-decoration:none;}
.Navbar_Item a {color:black; text-decoration:none;}
-
.Navbar_Item > ul {display:none; width:500px; height:80px; text-align:center; color:black;}
+
.Navbar_Item > ul {display:none; width:900px; height:80px; text-align:center; color:black; z-index:1100; }
-
.Navbar_Item > ul > li {float:left; list-style:none; width:100px; text-align:center; background-color:transparent; position:relative; top:10px;}
+
.Navbar_Item > ul > li {float:left; list-style:none; text-align:center; background-color:transparent; position:relative; top:10px; padding:0 10px;}
-
.Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000}
+
.Navbar_Item:hover{ border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;}
-
.Navbar_Item:hover > ul{ display:block;}
+
.Navbar_Item:hover > ul{zoom:1; display:block;}
-
.Navbar_Item:hover >a {color:#D00000}
+
.Navbar_Item:hover >a {color:#D00000; background-color:#fafaf8;}
-
.Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000}
+
.Navbar_Item > ul > li:hover {border-bottom:1px solid #D00000; color:#D00000; background-color:#fafaf8;}
.Navbar_Item > ul > li:hover >a {color:#D00000}
.Navbar_Item > ul > li:hover >a {color:#D00000}
 +
.BackgroundofSublist{position:absolute; left:-1000px; width:2000px; height:80px; background-color:#ffffff; opacity:0;}
#Home_Sublist{position:relative; top:0px; left:-50px;}
#Home_Sublist{position:relative; top:0px; left:-50px;}
-
#Team_Sublist{position:relative; top:0px; left:-60px;}
+
#Team_Sublist{position:relative; top:0px; left:-110px;}
-
#Project_Sublist{position:relative; top:0px; left:-200px;}
+
#Project_Sublist{position:relative; top:0px; left:-180px;}
#Model_Sublist{position:relative; top:0px; left:-140px;}
#Model_Sublist{position:relative; top:0px; left:-140px;}
-
#Lab_Sublist{position:relative; top:0px; left:-180px;}
+
#DataPage_Sublist{position:relative; top:0px; left:-60px;}
-
#HumanPractice_Sublist{position:relative; top:0px; left:-220px;}
+
#Safety_Sublist{position:relative; top:0px; left:-180px;}
 +
#HumanPractice_Sublist{position:relative; top:0px; left:-470px;}
-
#iGEM_logo{position:relative; top:8px; float:right; height:120px;}
+
#iGEM_logo{position:absolute; top:30px; left:1090px; height:80px;}
/*end navigation bar*/
/*end navigation bar*/
/*Major body*/
/*Major body*/
-
#MajorBody{position:absolute; top:0px; left:0px; width:1350px; height:1019px;}
+
#MajorBody{position:absolute; top:0px; left:0px; width:1200px; height:1380px; background-color:#f9f9f7;}
-
#ProjectTitle{position:absolute; left:0px; background-color:#cf0000; width:1350px; height:210px; top:150px; z-index:600;}
+
#ProjectTitle{position:absolute; left:0px; width:1200px; height:184px; top:150px; z-index:600;}
-
#ProjectName{position:absolute; left:420px; top:30px; border-bottom:0px; color:#FFFFFF; font-size:65px; font-family:Impact,Arial, Helvetica, sans-serif;}
+
#TitleBackground{position:absolute; left:0px; top:0px; width:1200px;}
-
#ProjectSubname{position:absolute; left:300px; top:130px; border-bottom:0px; color:#FFFFFF; font-size:19px;  font-family:Arial, Helvetica, sans-serif;}
+
#LittleScout{position:relative; left:0px; top:-3px; width:70px;}
-
#Major_background{position:fixed; top:130px; width:1350px; z-index:-1;}
+
#ProjectName{position:absolute; left:405px; top:20px; border-bottom:0px; color:#FFFFFF; font-size:48px; font-family:calibri,Arial, Helvetica, sans-serif;}
 +
#ProjectSubname{position:absolute; left:0px; width:1200px; top:105px; border-bottom:0px; color:#FFFFFF; font-size:18px;  font-family: calibri， Arial, Helvetica, sans-serif; text-align:center;}
-
#AbstractBox{position:absolute; left:490px; top:410px; width:770px; height:590px; background-color:transparent;}
+
#MajorBodyBackground{position:absolute; top:334px; left:0px; width:1200px;}
-
#AbstractBackground{width:100%; height:100%; background-color:#FFFFFF; opacity:0.5;}
+
 
-
#AbstractContent{position:absolute; left:30px; top:30px; width:710px; font-size:20px; font-family:Arial, Helvetica, sans-serif; text-align:justify;}
+
#PreLocation{font-size:14px; font-family:arial,helvetica,sans-serif; color:#ca4321;position:absolute;top:190px;left:0px;text-align:center;width:1200px;line-height:20px; font-weight:bold;}
-
#AbstractContent:first-letter{font-size:50px;}
+
 
-
.SmallBoxes{width:370px; height:280px;background-color:#FFFFFF}
+
#AbstractBox{position:absolute; left:430px; top:390px; width:710px; height:540px; background-color:transparent;}
-
#AppendixBox1{position:absolute; left:90px; top:410px;}
+
#AbstractBackground{width:100%; height:100%; background-color:#FFFFFF; opacity:0.8;}
-
#AppendixBox2{position:absolute; left:90px; top:720px;}
+
#AbstractContent{position:absolute; left:40px; top:25px; width:630px; font-size:18px; line-height:25px ; font-family: calibri, Arial, sans-serif; text-align:justify;}
-
#AppendixBox3{position:absolute; left:90px; top:1030px;}
+
#AbstractContent:first-letter{font-size:30px;}
-
#AppendixBox4{position:absolute; left:490px; top:1030px;}
+
.SmallBoxes{width:340px; height:250px;background-color:#FFFFFF; overflow:hidden;font-family: calibri， Arial, Helvetica, sans-serif; }
-
#AppendixBox5{position:absolute; left:890px; top:1030px;}
+
.SmallBoxes > img{position:absolute; left:0px; top:0px;}
 +
.SmallBoxes p{position:absolute; left:20px; top:75px; width:300px; font-size:14px; color:#ffffff; text-align:justify;}
 +
.SmallBoxes a{position:absolute; left:240px; top:220px; width:300px; font-size:12px; color:#ffffff; text-align:justify;}
 +
 
 +
 
 +
#AppendixBox1{position:absolute; left:60px; top:390px; background-color:#aa4630;}
 +
#Appendix1Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix1Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox2{position:absolute; left:60px; top:680px; background-color:#e98d70;}
 +
#Appendix2Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix2Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox3{position:absolute; left:60px; top:970px; background-color:#f37021;}
 +
#Appendix3Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix3Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox4{position:absolute; left:430px; top:970px; background-color:#1f8a70;}
 +
#Appendix4Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix4Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
#AppendixBox5{position:absolute; left:800px; top:970px; background-color:#004258;}
 +
#Appendix5Title{position:absolute; left:0px; top:70px; width:340px; text-align:center; color:#ffffff; font-size:16px; border-bottom:0px; line-height:28px;}
 +
#Appendix5Cover{position:absolute; left:0px; top:170px; width:370px; height:280px;}
 +
 
 +
.AppendixHeadLine{position:absolute; left:20px; top:20px; width:250px;border-bottom:0px; font-size:23px; color:#ffffff;}
 +
.AppendixIcon{position:absolute; left:270px; top:20px; width:50px; }
/*end of Major body*/
/*end of Major body*/
-
#AcknowledgementBox{ position:absolute; left:0px; top:1380px; width:1350px;}
+
#AcknowledgementBox{ position:absolute; left:0px; top:1280px; width:1200px;}
Line 184: Line 210:
<div id="navigationblock">
<div id="navigationblock">
<img id="navbarlogo" src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg"/>
<img id="navbarlogo" src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg"/>
 +
       
<ul id="navigationbar">
<ul id="navigationbar">
<li id="PKU_navbar_Home" class="Navbar_Item">
<li id="PKU_navbar_Home" class="Navbar_Item">
-
<a href="https://2013.igem.org/Team:Peking">home</a>
+
                       
 +
<a href="https://2013.igem.org/Team:Peking">Home</a>
<ul id="Home_Sublist" >
<ul id="Home_Sublist" >
-
<li><a>Item1</a></li>
 
-
<li><a>Item2</a></li>
 
-
<li><a>Item3</a></li>
 
</ul>
</ul>
</li>
</li>
<li id="PKU_navbar_Team" class="Navbar_Item">
<li id="PKU_navbar_Team" class="Navbar_Item">
-
<a href="https://2013.igem.org/Team:Peking/Team">team</a>
+
<a href="">Team</a>
<ul id="Team_Sublist">
<ul id="Team_Sublist">
-
<li><a>Item1</a></li>
+
                                <div class="BackgroundofSublist"></div>
-
<li><a>Item2</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
-
<li><a>Item3</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Team/Attributions">Attributions</a></li>
</ul>
</ul>
</li>
</li>
<li id="PKU_navbar_Project" class="Navbar_Item">
<li id="PKU_navbar_Project" class="Navbar_Item">
-
<a href="https://2013.igem.org/Team:Peking/Project">project</a>
+
<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
<ul id="Project_Sublist">
<ul id="Project_Sublist">
-
<li><a>Existing Biosensors</a></li>
+
                                <div class="BackgroundofSublist"></div>
-
<li><a>Plug-ins & Expansion</a></li>
+
                                <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
-
<li><a>Band-pass Circuit</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
-
                                 <li><a>Application</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
 +
                                 <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
</ul>
</ul>
</li>
</li>
<li id="PKU_navbar_Model" class="Navbar_Item">
<li id="PKU_navbar_Model" class="Navbar_Item">
-
<a href="https://2013.igem.org/Team:Peking/Model">model</a>
+
<a href="">Model</a>
<ul id="Model_Sublist">
<ul id="Model_Sublist">
-
<li><a>Item1</a></li>
+
                                <div class="BackgroundofSublist"></div>
-
<li><a>Item2</a></li>
+
                                <li><a href="https://2013.igem.org/Team:Peking/Model">Band-pass Filter</a></li>
-
<li><a>Item3</a></li>
+
                                <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
</ul>
</ul>
</li>
</li>
-
<li id="PKU_navbar_Lab" class="Navbar_Item">
+
                        <li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:90px">
-
<a href="https://2013.igem.org/Team:Peking/Lab">safty</a>
+
<a href="">Data page</a>
-
<ul id="Lab_Sublist">
+
<ul id="DataPage_Sublist">
-
<li><a>Item1</a></li>
+
                                <div class="BackgroundofSublist"></div>
-
<li><a>Item2</a></li>
+
                                <li><a href="https://2013.igem.org/Team:Peking/DataPage/Parts">Parts</a></li>
-
<li><a>Item3</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
 +
 
 +
</ul>
 +
</li>
 +
<li id="PKU_navbar_Safety" class="Navbar_Item">
 +
<a href="https://2013.igem.org/Team:Peking/Safety">Safety</a>
 +
<ul id="Safety_Sublist">
</ul>
</ul>
</li>
</li>
<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px">
<li id="PKU_navbar_HumanPractice" class="Navbar_Item" style="width:120px">
-
<a href="https://2013.igem.org/Team:Peking/HumanPractice">human practice</a>
+
<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
-
<ul id="HumanPractice_Sublist">
+
<ul id="HumanPractice_Sublist">
-
<li><a>Item1</a></li>
+
                                <div class="BackgroundofSublist"></div>
-
<li><a>Item2</a></li>
+
                                <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire Survey</a></li>
-
<li><a>Item3</a></li>
+
<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li>
 +
                                <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
 +
<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
 +
 
</ul>
</ul>
</li>
</li>
</ul>
</ul>
-
         <a href="https://igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
+
         <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
</div>
</div>
<!--end navigationbar-->
<!--end navigationbar-->
<!--MajorBody-->
<!--MajorBody-->
-
<div id="MajorBody">
+
<div id="MajorBody">
-
        <img id="Major_background" src="https://static.igem.org/mediawiki/igem.org/4/47/Peking_hompage_Background.jpg"> 
+
<div id="ProjectTitle">
<div id="ProjectTitle">
-
<h1 id="ProjectName">AROMATICS BUSTED</h1>
+
                <img id="TitleBackground" src="https://static.igem.org/mediawiki/igem.org/c/c5/Peking2013_home_title.jpg" />
-
                 <h1 id="ProjectSubname">A FAST, EASY AND ACCURATE METHOD TO DETECT TOXIC AROMATIC COMPOUNDS</h1>
+
               
 +
<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
 +
                 <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
 +
 +
<p id="PreLocation">
 +
<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
 +
      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
 +
</p>
 +
 
 +
 
 +
 
</div>
</div>
 +
        <img id="MajorBodyBackground" src="https://static.igem.org/mediawiki/igem.org/9/97/Peking2013_Home_background.jpg" />
         <div id="AbstractBox">
         <div id="AbstractBox">
             <div id="AbstractBackground"></div>
             <div id="AbstractBackground"></div>
-
            <p id="AbstractContent">As aromatic pollution levels rise, and along with it the environmental and health hazards that it presents, finding a better way of detecting these aromatic compounds becomes more and more important. Some prokaryotes, including <I>Escherichia coli</I> and <I>Pseudomonas putida</I>, naturally produce proteins capable of both detecting toxic aromatic molecules and regulating the transcription of corresponding catabolic genes. However, these naturally existing biosensors are limited by their detection range, expression leakage, and induction ratio.<br/><br/>Using these proteins, our team designed a series of improved aromatic sensors and combined them with related enzymes from their original catabolic pathways to increase the molecule detection range of these sensors. In order to semi-quantitatively measure the concentrations of target compounds, we constructed a biological band-pass circuit, enabling our sensors to detect the concentrations of target compounds within a certain range. In sum, we have been working on a fast, easy and accurate way to detect toxic aromatic compounds.</p>
+
              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.
 +
<br/><br/>
 +
Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.  
 +
<br/><br/>
 +
Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.  
 +
<br/><br/>
 +
We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
 +
</p>
         </div>
         </div>
-
         <div id="AppendixBox1" class="SmallBoxes">
+
         <div id="AppendixBox1" class="SmallBoxes" >
 +
              <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
 +
              <div id="Appendix1Cover" >
 +
                    <img id="AppendixImg1" src="https://static.igem.org/mediawiki/igem.org/e/ec/Peking2013_homepage_appendix1.jpg" />
 +
                    <h1 class="AppendixHeadLine">Biosensor Mining</h1>
 +
                    <img id="Appendix1Icon" class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/1/13/Peking2013_home_appendix1icon.png" />
 +
                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
 +
              </div>
         </div>
         </div>
         <div id="AppendixBox2" class="SmallBoxes">
         <div id="AppendixBox2" class="SmallBoxes">
 +
              <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
 +
              <div id="Appendix2Cover" >
 +
                    <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
 +
                    <h1 class="AppendixHeadLine">Biosensors</h1>
 +
                    <img id="Appendix2Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
 +
                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
 +
              </div>
         </div>
         </div>
         <div id="AppendixBox3" class="SmallBoxes">
         <div id="AppendixBox3" class="SmallBoxes">
 +
              <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
 +
              <div id="Appendix3Cover" >
 +
                    <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
 +
                    <h1 class="AppendixHeadLine">Adaptors</h1>
 +
                    <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
 +
                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
 +
              </div>
         </div>
         </div>
         <div id="AppendixBox4" class="SmallBoxes">
         <div id="AppendixBox4" class="SmallBoxes">
 +
              <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
 +
              <div id="Appendix4Cover" >
 +
                    <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
 +
                    <h1 class="AppendixHeadLine">Band-pass Filter</h1>
 +
                    <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
 +
                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
 +
              </div>
         </div>
         </div>
         <div id="AppendixBox5" class="SmallBoxes">
         <div id="AppendixBox5" class="SmallBoxes">
 +
              <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
 +
              <div id="Appendix5Cover" >
 +
                    <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
 +
                    <h1 class="AppendixHeadLine">Human Practice</h1>
 +
                    <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
 +
                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
 +
              </div>
         </div>         
         </div>         
Line 269: Line 363:
<script type="text/javascript">
<script type="text/javascript">
$(window).scroll(function(){
$(window).scroll(function(){
-
   if(0!=$(window).scrollLeft()){ $('#navigationblock').css('left',-$(window).scrollLeft());}
+
   if(0==document.getElementById("content").offsetLeft){ $('#navigationblock').css('left',-$(window).scrollLeft());}
 +
  else{$('#navigationblock').css('left',document.getElementById("content").offsetLeft); }
});
});
 +
 +
$(window).resize(function(){
 +
  if(0==document.getElementById("content").offsetLeft){ $('#navigationblock').css('left',-$(window).scrollLeft());}
 +
  else{$('#navigationblock').css('left',document.getElementById("content").offsetLeft); }
 +
});
 +
 +
document.getElementById("AppendixBox1").onmouseover=function(){MoveInSlide("#Appendix1Cover")};
 +
document.getElementById("AppendixBox1").onmouseout=function(){MoveOutSlide("#Appendix1Cover")};
 +
document.getElementById("AppendixBox2").onmouseover=function(){MoveInSlide("#Appendix2Cover")};
 +
document.getElementById("AppendixBox2").onmouseout=function(){MoveOutSlide("#Appendix2Cover")};
 +
document.getElementById("AppendixBox3").onmouseover=function(){MoveInSlide("#Appendix3Cover")};
 +
document.getElementById("AppendixBox3").onmouseout=function(){MoveOutSlide("#Appendix3Cover")};
 +
document.getElementById("AppendixBox4").onmouseover=function(){MoveInSlide("#Appendix4Cover")};
 +
document.getElementById("AppendixBox4").onmouseout=function(){MoveOutSlide("#Appendix4Cover")};
 +
document.getElementById("AppendixBox5").onmouseover=function(){MoveInSlide("#Appendix5Cover")};
 +
document.getElementById("AppendixBox5").onmouseout=function(){MoveOutSlide("#Appendix5Cover")};
 +
 +
function MoveInSlide(SlideId)
 +
{
 +
$(SlideId).stop().animate({top:"0px"},"fast");
 +
         
 +
};
 +
 +
 +
 +
function MoveOutSlide(SlideId)
 +
{
 +
$(SlideId).stop().animate({top:"170px"},"fast");
 +
       
 +
};
 +
 +
</script>
</script>
</html>
</html>

Latest revision as of 18:10, 28 October 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment

★Presentation: Room 34-101, Saturday 12:30 PM, Session 2     ★Poster: Sunday, #13 (Stata)

Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.

Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.

Mining aromatics-sensing Biobricks
from the genomic database

Biosensor Mining

The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot.

LEARN MORE

High-performance, profile-
specific biosensors

Biosensors

A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.

LEARN MORE

Converting the undetectable into
the detectable

Adaptors

To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.

LEARN MORE

Rapidly determining
the analyte concentration

Band-pass Filter

For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples.

LEARN MORE

Communications, Questionnaire survey,
Factory Visit and interview

Human Practice

To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.

LEARN MORE

Retrieved from "http://2013.igem.org/Team:Peking"