Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog

From 2013.igem.org

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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: <u> '''Small Phage''' </u> </font>
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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<font size="4"> '''8/7/13''' </font>
<font size="4"> '''8/7/13''' </font>
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- Performed phage titer determination for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]].
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- Performed spot test to estimate phage titerfor [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]].
- Phage Purification Group started the purification process with CsCl gradient.
- Phage Purification Group started the purification process with CsCl gradient.
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<font size="4"> '''7/31/13''' </font>
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<font size="4"> '''8/9/13''' </font>
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- Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]].
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- Performed Preliminary Titer for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]].
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<font size="4"> '''8/11/13''' </font>
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- Started approximately 20mL of E coli B liquid culture overnight.
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<font size="4"> '''8/12/13''' </font>
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- Performed titer - repeat for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]] based on the results from 8.10 preliminary titer test.
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- Started T1 propagation.
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- Made accurate x2 top agar as preparation for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]].
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<font size="4"> '''8/13/13''' </font>
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- Started approximately 20mL of E coli B liquid culture overnight.
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<font size="4"> '''8/14/13''' </font>
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- Performed mutagenesis and spot test for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.14_Mutagen_Concentration_Test_-_Seventh_Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]].
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- Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.
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<font size="4"> '''8/15/13''' </font>
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- T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 10<sup>9</sup> pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration.
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- Started approximately 30mL of E coli B liquid culture overnight.
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<font size="4"> '''8/16/13''' </font>
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- Started [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]].
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- Phage Purification team performed the CsCl gradient for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.14_Mutagen_Concentration_Test_-_Seventh_Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]].
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- Started approximately 10 mL of E coli B liquid culture overnight.
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{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 15:37, 9 September 2013


Small Phage July - August Notebook: August 1 - August 16 Daily Log



Small Phage
March-April
May-June
July-August
September-October

8/1/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.


8/2/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.


8/3/13

- E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.


8/4/13

- Started E coli liquid culture over night


8/5/13

- Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Discussed ideas for modeling phage plaque sizes


8/6/13

- Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Started 5mL of E coli B liquid culture overnight.


8/7/13

- Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.

- Phage Purification Group started the purification process with CsCl gradient.


8/8/13

- Started approximately 15mL of E coli B liquid culture overnight.


8/9/13

- Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.


8/11/13

- Started approximately 20mL of E coli B liquid culture overnight.


8/12/13

- Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test.

- Started T1 propagation.

- Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.


8/13/13

- Started approximately 20mL of E coli B liquid culture overnight.


8/14/13

- Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol.

- Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.


8/15/13

- T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 109 pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration.

- Started approximately 30mL of E coli B liquid culture overnight.


8/16/13

- Started 8.16 Modeling Phage Plaque Sizes - Experiment One.

- Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol.

- Started approximately 10 mL of E coli B liquid culture overnight.



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