"
15/07/13
From 2013.igem.org
(Difference between revisions)
AliceHaworth (Talk | contribs) (→Count colonies on agar plates) |
AliceHaworth (Talk | contribs) |
||
| Line 33: | Line 33: | ||
Therefore there was 1.2x10^8 cfu/ug | Therefore there was 1.2x10^8 cfu/ug | ||
| + | |||
| + | |||
| + | ==Replate to get individual colonies== | ||
| + | |||
| + | *Take an individual colonies | ||
| + | *Streak out onto new plate | ||
| + | *Sterilize loop using bunsen burner | ||
| + | *Incubate at 37 overnight | ||
Latest revision as of 10:30, 14 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Count colonies on agar plates
| Plate | Colonies |
| Viability control | Covered |
| Negative control | 1 contaminant |
| 20ul of 50pg/ul | 135 |
| 20ul of 10pg/ul | 96 |
| 200ul of 50pg/ul | ~1600 |
| 200ul of 10pg/ul | 944 |
Therefore there was 1.2x10^8 cfu/ug
Replate to get individual colonies
- Take an individual colonies
- Streak out onto new plate
- Sterilize loop using bunsen burner
- Incubate at 37 overnight
