Exeter/7 August 2013

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Latest revision as of 19:56, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Glycerol stocks

200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
Store at -80 degrees celsius
Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow

We miniprepped:

[http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP

[http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM

[http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM

[http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM

[http://parts.igem.org/Part:BBa_J04450 RFP control] CAM

3 NEB (RFP and Terminator) AMP

[http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM

NEB4 (RBS and cph8) AMP

NEB1 (RBS and cph8) AMP

[http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM

[http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM

Adam and Tom

Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli

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+{{:Team:Exeter/Template/Header}}
+<div class="container">
+    <div class="row">
+        <div class="span" style="text-align:justify">
 Glycerol stocks
 *200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
@@ Line 4: / Line 9: @@
 *Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow
-We miniprepped:
+==We miniprepped:==
-* B0034 (RBS) AMP
-* K592010 (yellow) CAM
+* [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP
-* S05058 (RBS + yellow) CAM
+* [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM
-* K608002 (Promoter and RBS) CAM
+* [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM
-* RFP control CAM
+* [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM
+* [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM
 * 3 NEB (RFP and Terminator) AMP
-* K592012 (Magenta) CAM
+* [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM
 * NEB4 (RBS and cph8) AMP
@@ Line 25: / Line 31: @@
 * NEB1 (RBS and cph8) AMP
-* B0015 (Terminator) CAM
+* [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM
-* K592018 (RBS + Cyan) CAM
+* [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM
 == Adam and Tom ==
@@ Line 34: / Line 39: @@
 Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)
+Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
+   </div>
+</div>
+{{:Team:Exeter/Template/Footer}}