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Team:DTU-Denmark/Notebook/6 July 2013
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=208 lab= | =208 lab= | ||
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==Procedure== | ==Procedure== | ||
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| - | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html | + | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though. |
Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | Then, viewed the periplasmic and cytoplasmic cell contents under UV light. | ||
Latest revision as of 20:39, 16 September 2013
6 July 2013
Contents |
208 lab
Main purposes today
Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
Who was in the lab
Kristian, Helen
Procedure
Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
Conclusion from today
The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
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