18/07/13

From 2013.igem.org

(Difference between revisions)
(Replaced content with "==Double digest==")
(→Single digest)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
==Double digest==
+
==Single digest==
 +
*Digesting RFP plasmid again using a single restriction enzyme- XbaI
 +
*Digesting DNA samples 1-6
 +
*200ng of DNA is run
 +
*Volumes for digest:
 +
{| border=1
 +
|Sample nr||1||2||3||4||5||6
 +
|-
 +
|DNA||0.7||0.7||0.8||1.3||1.2||2.6
 +
|-
 +
|Buffer||2||2||2||2||2||2
 +
|-
 +
|Water||16.3||16.3||16.2||15.7||15.8||14.4
 +
|-
 +
|XbaI||1||1||1||1||1||1
 +
|}
 +
*Buffer used was cutsmart
 +
*Incubate samples at 37C for 1h
 +
*2ul of orange G dye was added to each samples
 +
*Samples were then frozen overnight to run on gel on Monday

Latest revision as of 14:16, 22 August 2013

Single digest

  • Digesting RFP plasmid again using a single restriction enzyme- XbaI
  • Digesting DNA samples 1-6
  • 200ng of DNA is run
  • Volumes for digest:
Sample nr123456
DNA0.70.70.81.31.22.6
Buffer222222
Water16.316.316.215.715.814.4
XbaI111111
  • Buffer used was cutsmart
  • Incubate samples at 37C for 1h
  • 2ul of orange G dye was added to each samples
  • Samples were then frozen overnight to run on gel on Monday
Retrieved from "http://2013.igem.org/18/07/13"