"
Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period3/Dailylog
From 2013.igem.org
| (3 intermediate revisions not shown) | |||
| Line 16: | Line 16: | ||
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
| - | : | + | <font size = "4"> |
| + | |||
| + | : <u> '''Small Phage''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]] | ||
| Line 108: | Line 110: | ||
- Performed titer - repeat for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]] based on the results from 8.10 preliminary titer test. | - Performed titer - repeat for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Preliminary Experiments]] based on the results from 8.10 preliminary titer test. | ||
| - | - Started T1 propagation | + | - Started T1 propagation. |
| + | |||
| + | - Made accurate x2 top agar as preparation for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]]. | ||
<br> | <br> | ||
| Line 136: | Line 140: | ||
<font size="4"> '''8/16/13''' </font> | <font size="4"> '''8/16/13''' </font> | ||
| - | - Started [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]] | + | - Started [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling Phage Plaque Sizes - Experiment One]]. |
| + | |||
| + | - Phage Purification team performed the CsCl gradient for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.14_Mutagen_Concentration_Test_-_Seventh_Protocol|8.14 Mutagen Concentration Test - Seventh Protocol]]. | ||
| + | |||
| + | - Started approximately 10 mL of E coli B liquid culture overnight. | ||
<br> | <br> | ||
Latest revision as of 15:37, 9 September 2013
| ||
|
|
8/1/13 - Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
8/2/13 - Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration. - Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration. - Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/3/13 - E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/4/13 - Started E coli liquid culture over night
8/5/13 - Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Discussed ideas for modeling phage plaque sizes
8/6/13 - Check up on the phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Started 5mL of E coli B liquid culture overnight.
8/7/13 - Performed spot test to estimate phage titerfor 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments. - Phage Purification Group started the purification process with CsCl gradient.
8/8/13 - Started approximately 15mL of E coli B liquid culture overnight.
8/9/13 - Performed Preliminary Titer for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments.
8/11/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/12/13 - Performed titer - repeat for 8.2 Modeling Phage Plaque Sizes - Preliminary Experiments based on the results from 8.10 preliminary titer test. - Started T1 propagation. - Made accurate x2 top agar as preparation for 8.16 Modeling Phage Plaque Sizes - Experiment One.
8/13/13 - Started approximately 20mL of E coli B liquid culture overnight.
8/14/13 - Performed mutagenesis and spot test for 8.14 Mutagen Concentration Test - Seventh Protocol. - Performed spot test for T1 propagation. Spotted 5uL of -2 through -7 dilutions.
8/15/13 - T1 propagation revealed plaques on each of the tested dilutions, suggesting that this propagated T1 phage stock has a titer of at least 109 pfu/mL -> more accurate spot test / titer needed to determine the exact phage concentration. - Started approximately 30mL of E coli B liquid culture overnight.
8/16/13 - Started 8.16 Modeling Phage Plaque Sizes - Experiment One. - Phage Purification team performed the CsCl gradient for 8.14 Mutagen Concentration Test - Seventh Protocol. - Started approximately 10 mL of E coli B liquid culture overnight.
| |
