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20/08/13
From 2013.igem.org
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(Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...") |
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| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
| + | </div> | ||
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==Making nutrient agar plates and broth== | ==Making nutrient agar plates and broth== | ||
*Making up broth and agar solution to grow ''P. putida'' in. | *Making up broth and agar solution to grow ''P. putida'' in. | ||
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*Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth | *Freeze dried ''P. putida'' was rehydrated using 1ml of nutrient broth | ||
*The culture was aseptically transferred back into the broth containing tube | *The culture was aseptically transferred back into the broth containing tube | ||
| - | * | + | *Mix |
| - | *From that tube, colonies were streaked | + | *From that tube, colonies were streaked onto 6 plates and left to incubate over night at 30C |
==Growing bacteria in broth== | ==Growing bacteria in broth== | ||
Latest revision as of 09:18, 22 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Making nutrient agar plates and broth
- Making up broth and agar solution to grow P. putida in.
- For agar plates:
- 9.2g of nutrient agar medium
- 400ml of distilled water
- For broth:
- 3.2g of nutrient broth
- 400ml of distilled water
- Both were autoclaved for 45mins
- Agar was poured onto 16 plates
Plating out bacteria
- Freeze dried P. putida was rehydrated using 1ml of nutrient broth
- The culture was aseptically transferred back into the broth containing tube
- Mix
- From that tube, colonies were streaked onto 6 plates and left to incubate over night at 30C
Growing bacteria in broth
- 100ml of autoclaved broth was added to 2 flasks each
- The broth tube, containing the bacteria, was divided between flasks
- Flask were left to incubate at 30C on a shaker overnight
