Latest revision as of 16:46, 30 August 2013

Week 13

Monday

Many gels were run to check the validity of our results. We discovered that our gene number four ligation was not sucessful and we would have to repeat the ligation of that particular gene. We also ligated the L-arabinose promoters to the pump or GFP. This was done with the three strengths of the promoters: strong, medium, and weak.

Tuesday

The L-arabinose promoters (strong, medium, weak) were transformed with the GFP or the pump into E.coli for testing. These plates were then put in the incubator and should be checked tomorrow! We hope to see colonies and then check to see if the expression corresponds with the strength of the promoter.

Wednesday

The plates were checked, and unfortunately, no colonies were found. The plates were left in the incubator to see if more time would allow for growth.

Thursday

The plates were checked again, and no growth was found. We decided that this must mean either our transformation or ligation of these parts was not successful.

Friday

The lab was cleaned and our boxes were placed in storage. The title and abstract forms were also completed.

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 <br><FONT color = 'green' size="+20">Week 13</FONT><BR><BR>
    <H1 align = left>Monday</H1>
-   <p style="text-align:justify">Transformation efficiency of the DH5 alpha cells were checked, and the transformation was successful using the pUC19. 5 colonies were found on our plate as of about 8 pm. Our BioBrick parts in the standard plasmid (pSB1C3) were transformed into competent cells in hopes of miniprepping out the genes. </p><br>
+   <p style="text-align:justify">Many gels were run to check the validity of our results. We discovered that our gene number four ligation was not sucessful and we would have to repeat the ligation of that particular gene. We also ligated the L-arabinose promoters to the pump or GFP. This was done with the three strengths of the promoters: strong, medium, and weak.</p><br>
    <H1 align = left>Tuesday</H1>
-   <p style="text-align:justify">Growth of the transformed cells occurred!</p><br>
+   <p style="text-align:justify">The L-arabinose promoters (strong, medium, weak) were transformed with the GFP or the pump into E.coli for testing. These plates were then put in the incubator and should be checked tomorrow! We hope to see colonies and then check to see if the expression corresponds with the strength of the promoter.</p><br>
    <H1 align = left>Wednesday</H1>
-   <p style="text-align:justify">The mini prep protocol was completed with all 7 of our original genes. DNA was seen, and the next step would be to do PCR with the products and run a gel to confirm our results. </p><br>
+   <p style="text-align:justify">The plates were checked, and unfortunately, no colonies were found. The plates were left in the incubator to see if more time would allow for growth.</p><br>
    <H1 align = left>Thursday</H1>
-   <p style="text-align:justify">The PCR of the miniprepped genes occurred today. A gel still needs to be run to confirm we have the correct products before we continue.</p><br>
+   <p style="text-align:justify">The plates were checked again, and no growth was found. We decided that this must mean either our transformation or ligation of these parts was not successful. </p><br>
    <H1 align = left>Friday</H1>
-   <p style="text-align:justify">A gel was run to check the PCR that was run on Thursday, but we think the electrophoresis was ran too long because of notable band streaking. Also, we believe we destained the gel for too long because the bands were hardly visible. We hope to repeat this gel on Monday to verify the results. 20 agar plates were also made: 10 with chloramphenicol and 10 with kanamycin. </p><br>
+   <p style="text-align:justify">The lab was cleaned and our boxes were placed in storage. The title and abstract forms were also completed. </p><br>
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Team:MSOE Milwaukee/Week13

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