Team:Goettingen

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            <li><a href="#background"><span class="bkg">Background</span></a></li>
 
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<b>Site Map of Team Goettingen's wiki</b><br />
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<p><strong><a href="https://2013.igem.org/Team:Goettingen/Project">Project</a></strong></p>
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  <li> <a href="https://igem.org/Login" target="_blank" style="color:#596678">Log in to iGEM account</a></li>
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<ul>
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<li><a href="https://2013.igem.org/Team:Goettingen/Project/OurProject">Project Overview</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Team/Reporter">Reporter Team</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Team/Array">Array Team</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Team/DAC">DAC Team</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Project/Outlook">Outlook</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Team/Organization">Organization Team</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Project/Collaborations">Collaborations</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Parts">Parts</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/Safety">Safety</a></li>
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<p><strong><a href="https://2013.igem.org/Team:Goettingen/Team">Team</a></li></strong></p>
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<li><a href="https://2013.igem.org/Team:Goettingen/Team/Instructors">Instructors</a></li>
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<p><strong><a href="https://2013.igem.org/Team:Goettingen/NoteBook">Notebook</a></strong></p>
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<li><a href="https://2013.igem.org/Team:Goettingen/NoteBook/timeline">Timeline</a></li>
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<li><a href="https://static.igem.org/mediawiki/2013/e/eb/Goe-iGEM_Protocolls.pdf">Protocol</a></li>
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<li><a href="https://2013.igem.org/Team:Goettingen/NoteBook/Acknowlegement">Acknowlegement</a></li>
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    <h1>The beast and its Achilles heel:<br / >  
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        A novel target to fight multi-resistant pathogenic bacteria </h1>
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<p><strong><a href="https://2013.igem.org/Team:Goettingen/HumanPractice">Human practice</a></strong></p>
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        <h2>The lack of novel antibiotics: the problem that we address </h2>
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        <p>The discovery of penicillin by Alexander Fleming in 1928 and the broad application of antibiotics marked a major victory of mankind in the  battle against infectious diseases. </p>
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<li><a href="https://2013.igem.org/Team:Goettingen/HumanPractice/PanelDiscussion">Panel discussion</a></li>
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<p> However, shortly after the beginning of industrial  penicillin  production in 1942 some human  pathogens  already acquired resistance against penicillin and related antibiotics. </p>
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<li><a href="https://2013.igem.org/Team:Goettingen/HumanPractice/BMBF_congress">BMBF congress</a></li>
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<p>Moreover, the frequent use of antibiotics in the past and the rapid adaptation of bacteria to  these compounds  led to the development  of many multi-resistant pathogens.  </p>
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<li>Interivews</li>
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<p>Some of the pathogenic  bacteria  are even resistant  against most commercially available antibiotics. Therefore, both the discovery and the development of new antibacterial substances  are extremely important to fight these threats of human health. Unfortunately, the pipeline of novel compounds with antibiotic activity  and appropriate pharmaceutical properties is  empty. This makes the challenge of finding  new compounds even more urgent!</p>
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<p> The first step in designing efficient novel antibacterial  compounds is the identification  of a suitable target. Very recently, a potentially interesting  target has been identified. The emerging signaling molecule cyclic dinucleotide c-di-AMP is essential in the Gram-positive model organism  B. subtilis  and in closely related pathogenic bacteria  such as Listeria monocytogenes, Streptococcus pneumoniae, and Staphylococcus aureus. </p>
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<li><a href="https://2013.igem.org/Team:Goettingen/HumanPractice/v1">Interview with Dr. Perl</a></li>
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<p> These bacteria cause serious diseases and are often multi-resistant. c-di-AMP is needed for  the control  of  vital cellular processes in these pathogens because both: lack and accumulation of c-di-AMP, strongly inhibit growth of the bacteria. </p>
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<li><a href="https://2013.igem.org/Team:Goettingen/HumanPractice/v2">Interview with Prof. Groß</a></li>
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<p> Therefore, any substance that disturbs  the homeostasis of the important signaling molecule c-di-AMP and the interaction with its  target is of substantial interest to fight human pathogens. The next important step in identifying  efficient antibacterial compounds, which either inhibit c-di-AMP biosynthesis or interfere  with the essential function of c-di-AMP  in the cell, is the development of a powerful screening system. </p>
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<li><a href="https://2013.igem.org/Team:Goettingen/HumanPractice/v3">Street Interview</a></li>
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<li><a href="https://docs.google.com/forms/d/1ZRpyowfl9TZgov878VbCtl26n6DnpvHY9Tx9FGTAPCI/viewform" target="_blank">Online-questionnaire</a></li>
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<h2>Our project: </h2>
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<p>Our project is aimed at the development of  a simple screening system, which allows the rapid identification and characterization of substances that disturb c-di-AMP homeostasis in pathogenic bacteria. </p>
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<p><strong><a href="https://2013.igem.org/Team:Goettingen/Achievement">Achievements</a></strong></p>
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<p> The principle of how such a screening  system could look like is illustrated in Figure 1.  The screening system will be established in the  non-pathogenic bacterium E. coli. </p>
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<p> First, we want to construct a promoter-reporter gene fusion which allows us to monitor  the activity of a transcription factor that only binds to a specific DNA sequence (operator) in the presence of c-di-AMP. The operator sequence will be placed between a constitutively  active promoter and a reporter gene, such as  lacZ  and  gfp  encoding the -galactosidase and  the green-fluorescent protein (GFP), respectively. </p>
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<p> The activity of either of the two proteins is very easy to detect. Then, we will evaluate  whether binding of the transcription factor and  thus inhibition of the promoter-reporter gene  fusion can be controlled by exogenous c-di-AMP. </p>
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<p> Finally, we want to express a diadenylate cyclase  from  B. subtilis  to inhibit the promoter reporter gene fusion by endogenously synthesized c-di-AMP. There are two big advantages of using E. coli as a host for the development of a screening system to  identify antibacterial compounds that interfere  with c-di-AMP homoeostasis in Gram-positive pathogenic bacteria. </p>
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<p> First, c-di-AMP is not synthesized in  E. coli. Thus, compounds that inhibit c-di-AMP synthesis will specifically inhibit growth of Gram-positive bacteria. Second, the use of a nonpathogenic E. coli strain, which is easy to cultivate will keep the costs very low.</p>
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<p>   We are confident that our screening system will facilitate the identification of novel  antibacterial substances because any change  in the activity of the c-di-AMP-dependent  promoter-reporter gene fusion, either by inhibition of c-di-AMP synthesis or by activation  of DNA-binding activity of the transcription  factor  will indicate perturbation of c-di-AMP homeostasis.  </p>
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<div style="font-size:8pt;margin-left:20px;margin-bottom:15pxz-index:2">Created by <a href="http://www.oneplusyou.com">OnePlusYou</a></div>
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<br />
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<img src="https://static.igem.org/mediawiki/2013/5/51/Goe-greenColi-labcoat.png" height="300" style="left: 60px;position: absolute;">
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  <div class="t-box bb br" style="
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  <h3>Project</h3>
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  <p>Our project relates to the growing threat of antibiotic resistance in bacteria. In order to fight this, we built a screening system targeting c-di-AMP (signaling molecule in Gram-positive bacteria). In other words: We developed an “Antibiotic Detector”!</p>
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<a class="moreinfo fr" href="/Team:Goettingen/Project" style="
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">MORE INFO</a>
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  <div class="t-box bb" style="
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  <h3>Team</h3>
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  <p>Our team consists of 12 ambitious and dedicated master students from the Georg-August University of Göttingen. We love biology, we care about the world, and of course we are super hot.</p><br><a class="moreinfo fr" href="/Team:Goettingen/Team" style="
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  <h3>Fun</h3>
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  <p>We are the ones who know how to have fun when PCRs don’t work, when proteins refuse to be expressed, when gels are a total mess and when the deadline is just about to hit us.</p><a class="moreinfo" href="Javascript:startComic()" style="
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  <h3>Outreach</h3>
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  <p>Here you can find all our related documents, including our booklet for fundraising, the slides of our presentations, progress reports, posters and flyers.</p><a class="moreinfo" href="/Team:Goettingen/Outreach" style="
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<div class="h-box fr" style="height:160px;padding:10px 20px;background:transparent;color:#eee">
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<strong style="font-size:13pt">Contact</strong><br>
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<b>E-mail:</b> igem2013@uni-goettingen.de<br />
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<b style="vertical-align:top">Address:</b> 
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<div style="background:transparent;display:inline-block">
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c/o  iGEM 2013<br />
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Department of General Microbiology<br />
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Grisebachstraße 8<br />
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37077 Göttingen<br />
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Germany<br />
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<strong style="font-size:13pt" class="fl">Sponsors</strong><br><br>
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<a href="https://2013.igem.org/Team:Goettingen/Team/Sponsors#kws"><img src="https://static.igem.org/mediawiki/2012/d/db/KWS_logo.png" style="height: 120px;display:inline"></a>
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&nbsp;&nbsp;&nbsp;
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<a href="https://2013.igem.org/Team:Goettingen/Team/Sponsors#uni-goe"><img src="https://static.igem.org/mediawiki/2013/4/4f/Goe-uni.png" style="height: 120px;display:inline"></a>
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&nbsp;&nbsp;&nbsp;
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<a href="https://2013.igem.org/Team:Goettingen/Team/Sponsors#gzmb"><img src="https://static.igem.org/mediawiki/2013/b/b8/Goe-GZMB.png" style="height: 120px;display:inline"></a>
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&nbsp;&nbsp;&nbsp;
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<div style="height:20px;width:100%;background:transparent"></div>
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<br><a href="https://2013.igem.org/Team:Goettingen/Team/Sponsors#G2L"><img src="https://static.igem.org/mediawiki/2013/1/10/Goe-g2l.PNG" style="height: 50px;display:inline"></a>
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<a href="https://2013.igem.org/Team:Goettingen/Team/Sponsors#UMG"><img src="https://static.igem.org/mediawiki/2013/8/8e/Goe-UMG.PNG" width="400' style=" padding-left:0px"="" style="height: 50px; width: auto;display: inline;/* float: right; */"></a>
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Latest revision as of 19:41, 3 October 2013








The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria


Site Map of Team Goettingen's wiki

Project

Team

Notebook

Human practice

Outreach

Achievements

Created by OnePlusYou

Project

Our project relates to the growing threat of antibiotic resistance in bacteria. In order to fight this, we built a screening system targeting c-di-AMP (signaling molecule in Gram-positive bacteria). In other words: We developed an “Antibiotic Detector”!

MORE INFO

Team

Our team consists of 12 ambitious and dedicated master students from the Georg-August University of Göttingen. We love biology, we care about the world, and of course we are super hot.


MORE INFO

Fun

We are the ones who know how to have fun when PCRs don’t work, when proteins refuse to be expressed, when gels are a total mess and when the deadline is just about to hit us.

MORE INFO

Outreach

Here you can find all our related documents, including our booklet for fundraising, the slides of our presentations, progress reports, posters and flyers.

MORE INFO

Contact
E-mail: igem2013@uni-goettingen.de
Address:
c/o iGEM 2013
Department of General Microbiology
Grisebachstraße 8
37077 Göttingen
Germany
Sponsors

           










Retrieved from "http://2013.igem.org/Team:Goettingen"