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Team:BYU Provo/Notebook/Phage Purification/Springexp/Period4/Dailylog
From 2013.igem.org
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Latest revision as of 00:04, 28 September 2013
| Phage Purification May - June Notebook: June 10 - June 23 Daily Log
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6/10/13 - Prepared RNase A for future use. - Prepared T4 and T7 cultures.
6/12/13 - Began first step of purification process using phage from 6.10 Phage Preparation.
6/14/13 - Worked on updating the website.
6/17/13 - Set up CsCl gradient 6.17 CsCl Gradient - Prepared T4 and T7 cultures.
6/19/13 - Began first step of purification process using phage from 6.17 Phage Preparation. - Ran spot tests on collected band from 6.17 CsCl Gradient.
6/20/13 - Finished 6.19 PEG Purification by centrifuging solution at 10,000 g 15 minutes at 4◦ C, removing supernatant, and suspending the pellet in phage suspension buffer.
6/21/13 We centrifuged the pellet at 10,000 g for 15 min at 4◦ C after suspending it to clean up the walls of the tube.
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