Team:Yale/Project Export
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<div id="header">{{Template:Team:Yale2013/Templates/Header}}</div> | <div id="header">{{Template:Team:Yale2013/Templates/Header}}</div> | ||
+ | <!-- Project Submenu adapted from cssmenumaker --> | ||
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+ | <div id="cssmenu1" style="width:730px; margin:0 auto;"> | ||
+ | <ul> | ||
+ | <li><a href='https://2013.igem.org/Team:Yale/Project_Overview'><span>Project Overview</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:Yale/Project_Validate'><span>Validate PLA Synthesis</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:Yale/Project_Bioassay'><span>Develop Bioassay</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:Yale/Project_MAGE'><span>Apply Mage</span></a></li> | ||
+ | <li class='active'><a href='https://2013.igem.org/Team:Yale/Project_Export'><span>Introduce Export System</span></a></li> | ||
+ | <li class='last'><a href='https://2013.igem.org/Team:Yale/Project_Bioplastic'><span>Make a Bioplastic</span></a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </html> | ||
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=== Introduce type 1 secretion system to export and extract PLA === | === Introduce type 1 secretion system to export and extract PLA === |
Revision as of 15:05, 23 September 2013
Introduce type 1 secretion system to export and extract PLA
- We needed a way to export the PLA once it was synthesized by the E. coli
- We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli
- She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
- This allowed the cells to export the PLA since the hlyA tag was attached to the granule
![PHA export system.jpg](/wiki/images/0/07/PHA_export_system.jpg)
- Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
![PLAandPHAexport.png](/wiki/images/f/f2/PLAandPHAexport.png)