Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Dailylog

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Revision as of 21:09, 13 September 2013

Phage Purification September - October Notebook: September 1 - September 14 Daily Log

9/4/13

- Went over the results of our T4 and T7 purification with the Large Phage and small phage group. - Updated wiki and discussed plans to have everything completed by the deadlines.

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9/6/13

Continued updating wiki.

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9/7/13

We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.

- 9.7 CsCl Gradient

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9/9/13

We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.

- 9.9 10X Phage Suspension Buffer Creation

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9/11/13

- We went ahead and ran another phage purification procedure with the new phage suspension buffer created in 9.9 10X Phage Suspension Buffer Creation

- 9.11 CsCl Gradient

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9/13/13

- We discussed the results from 9.11 CsCl Gradient and determined a new gradient to be run. Our plan is to run three gradients next class, one wild type and two mutant gradients.

Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8

Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6

Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6

- We also worked on our team profile and updated the notebook on the wiki.

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 - We discussed the results from [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] and determined a new gradient to be run.  Our plan is to run three gradients next class, one wild type and two mutant gradients.
-Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
+: Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
-Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6
+: Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6
-Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6
+: Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6
-We also worked on our team profile and updated the notebook on the wiki.
+- We also worked on our team profile and updated the notebook on the wiki.
 AB DL AC