Team:UNITN-Trento/Parts

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<!-- Ethylene -->

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<span class="title">Ethylene</span>

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<td class="code">BBa_K1065000</td>

<td class="name">EFE</td>

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Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate.

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<td class="code">BBa_K1065002</td>

<td class="name">EFE with double-terminator</td>

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EFE followed by the double terminator BBa_B0015.

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<td class="code">BBa_K1065001</td>

<td class="name">EFE arabinose-regulated</td>

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This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

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<!-- Methyl salicylate -->

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<span class="title">Methyl salicylate</span>

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<td class="code">BBa_K1065100</td>

<td class="name">BSMT1</td>

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This part encodes the BSMT1, it is the enzyme that methylates salycilic acid to obtain  methyl salycilate. It’s the original part  from the Eau de coli MIT iGEM 2006 project, resubmitted by us in pSB1C3.

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<td class="code">BBa_K1065103</td>

<td class="name">PchBA</td>

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PchA ,the enzyme catalyzing salicylate biosynthesis isochorismate, and PchB that is the isochorismate-pyruvate lyase for that form the precursor for the salycilic acid.

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<td class="code">BBa_K1065104</td>

<td class="name">SAM synthetase</td>

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This part encodes the SAM (S-AdoMet) synthetase gene, (which we friendly called "uncle SAM") . It is a methyl donor required to produce MeSA and other methylated molecules.

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<td class="code">BBa_K1065101</td>

<td class="name">BSMT1 arabinose-regulated</td>

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A methyltransferase (BSMT1) regulated by the araCpBAD inducible promoter.  

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<td class="code">Bba_K1065105</td>

<td class="name">SAM synthetase IPTG-regulated</td>

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This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

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<td class="code">BBa_K1065102</td>

<td class="name">PchBA IPTG-regulated and BSMT1 arabinose-regulated</td>

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This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter.

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<td class="code">BBa_K1065106</td>

<td class="name">BSMT1, PchBA and SAM synthetase</td>

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An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

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<!-- Blue light -->

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<span class="title">Blue light</span>

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<td class="code">Bba_K1065301</td>

<td class="name">amilCP IPTG-regulated</td>

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We attached pLac promoter to amilCP gene. This part does not work (sadly we saw no blue color). In fact, we started from BBa_K592009 which lacks the RBS (it is a coding part). <i>No RBS, no party!</i>

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<td class="code">Bba_K1065300</td>

<td class="name">BBa_K952003 with Yf1 and FixJ IPTG-regulated.</td>

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pLac+Yf1-FixJ-pFixK2-amil GFP without RBS and with pLac upstream.

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<td class="code">BBa_K1065303</td>

<td class="name">Yf1+FixJ+pFixK2</td>

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The starting point to build a blue-light sensible circuit: the photosensor, the response regulator with its promoter. This part was build merging BBa_K592016 and BBa_K592006.

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<td class="code">BBa_K1065310</td>

<td class="name">Yf1+FixJ+pFixK2+Inverter+pLambda+amilCP</td>

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This is a complete blue-light sensible circuit, inverted with cI. Yf1 and FixJ are under the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced.

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<td class="code">BBa_K1065302</td>

<td class="name">BBa_K1065300 improved</td>

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The same as BBa_K1065300: we performed a mutagenesis to add the missing RBS, now this works!!! It is: pLac+Yf1+FixJ+pFixK2+amilGFP.

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<td class="code">BBa_K1065305</td>

<td class="name">BBa_K952003 improved</td>

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The same as BBa_K952003: we performed a mutagenesis to add the missing RBS, now this works!!! It is: Yf1+FixJ+pFixK2+amilGFP.

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<td class="code">BBa_K1065306</td>

<td class="name">J23100+Yf1+FixJ</td>

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Yf1 and FixJ under the Anderson promoter (BBa_J23100).

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<td class="code">BBa_K1065204</td>

<td class="name">pXyl+EFE</td>

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EFE in pXyl vector, specific for B. subtilis.  

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<td class="code">BBa_K1065309</td>

<td class="name">BBa_K1065302+BBa_K1065002</td>

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Complete blue-light sensible circuit without inverter, producing both amilGFP and EFE.

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<!-- Bacillus -->

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<span class="title">Bacillus</span>

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<td class="code">BBa_K1065201</td>

<td class="name">pXyl+GFP</td>

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An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production.

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<td class="code">BBa_K1065106</td>

<td class="name">BSMT1, PchBA and SAM synthetase</td>

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A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840).

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<td class="code">BBa_K1065204</td>

<td class="name">pXyl+EFE</td>

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EFE in pXyl vector, specific for B. subtilis.  

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