19/09/13

(Difference between revisions)

Revision as of 10:08, 20 September 2013

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Isolating DNA using Omega nucleic acid purification kit and following its protocol.
Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
Selection was made by looking at gel pictures and selecting samples with visible bands.

Sample nr

Volume ul

Concentration ng/ul

260/280

260/230

Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

Preparing broths from individual colonie plates from previous day
Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

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+<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
+{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
+!align="center"|[[Team:Leicester|Home]]
+!align="center"|[[Team:Leicester/Team|Team]]
+!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
+!align="center"|[[Team:Leicester/Project|Project]]
+!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Leicester/Modeling|Modeling]]
+!align="center"|[[Team:Leicester/Notebook|Notebook]]
+!align="center"|[[Team:Leicester/Safety|Safety]]
+!align="center"|[[Team:Leicester/Attributions|Attributions]]
+|}
+</div>
 ==DNA isolation from PCR products==
 *Isolating DNA using Omega nucleic acid purification kit and following its protocol.