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Team:UGent/CloneManager
From 2013.igem.org
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<h1> Plasmids containing toxin ccdB</h1> | <h1> Plasmids containing toxin ccdB</h1> | ||
| + | <p> All of these plasmids are constucted using biobrick-standard parts: the plasmid backbones: pSB3T5, pSB4A5 and pSB6A1. | ||
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<h2>pSB3T5 T7 ccdB</h2> | <h2>pSB3T5 T7 ccdB</h2> | ||
<center><img src="https://static.igem.org/mediawiki/2013/4/4a/UGent_2013_PSB3T5-T7-ccdB.png" width="500"/></center> | <center><img src="https://static.igem.org/mediawiki/2013/4/4a/UGent_2013_PSB3T5-T7-ccdB.png" width="500"/></center> | ||
Revision as of 10:43, 21 September 2013
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To perform in silico analysis, we used Clone Manager. All plasmids were first implemented into the software, and then used to help with cloning simulation, restriction, PCR etc. Plasmid containing construct for chromosomal evolutionpTGD ccdA Pmb1 BCD7 GFP CmFRTThis plasmid contains the ccdA antitoxin and the reporter gene GFP. The homologous regions are necessary to perform Chemically Inducible Chromosomal evolution (CIChE). The removal of the chloramphenicol resistance cassette between the FTR sites is done using the method of Datsenko & Wanner [PNAS 2000].  Plasmids containing toxin ccdBAll of these plasmids are constucted using biobrick-standard parts: the plasmid backbones: pSB3T5, pSB4A5 and pSB6A1. pSB3T5 T7 ccdB pSB4A5 T7 ccdB pSB6A1 T7 ccdB New partpSB1C3 T7 ccdB
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