Team:Tokyo Tech

From 2013.igem.org

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<div align="right"><a href="">(go to Human Practice page)</a></div>
<div align="right"><a href="">(go to Human Practice page)</a></div>
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<a href=""><img src="https://static.igem.org/mediawiki/2013/8/83/Titech2013_home_human-practice-experiment.png" width="200"></a><br>
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<h4>[Fig1. Human Practice experiment]<br>
<h4>[Fig1. Human Practice experiment]<br>
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We did experiment class for high school students.
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<div align="right"><a href="">(see more)</a></div>
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<a href=""><img src="https://static.igem.org/mediawiki/2013/6/61/Titech2013_home_human-practice-enquete.png" width="200"></a><br>
<h4>[Fig2. Human Practice enquete]<br>
<h4>[Fig2. Human Practice enquete]<br>
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We did enqute in May festibal at University of Tokyo.
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Ninja is a Japan’s ancient warrior. Usually Ninja disguises himself as an ordinary <b>civilian</b> in public. Once he detects <b>Samurai</b> who is the assassination target, he immediately gets ready for battle. He defeats Samurai with <b>shuriken</b>, throwing knives.
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In our project, <i>E. ninja</i> heads the cast. <i>E. civilian</i> and <i>E. samurai</i> produce their own signal molecules. Depending on the received signal, <i>E. ninja</i> changes two states: <b>“mimic state”</b> and <b>“attack state”</b>. We aim to design the circuit that contains a bi-stable state and a signal dependent switching part.
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<div align="right"><a href="">(go to Story page)</a></div>
<div align="right"><a href="">(go to Story page)</a></div>
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However, in the case that uses both of signal molecules together, these signals induce a <b>crosstalk</b>. This crosstalk causes wrong state changing. To realize accurate switching, by network engineering, we have circumvented the crosstalk.
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<div align="right"><a href="">(go to State Changing page)</a></div>
<div align="right"><a href="">(go to State Changing page)</a></div>
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<a href=""><img src="https://static.igem.org/mediawiki/2013/6/61/Titech2013_home_human-practice-enquete.png" width="200"></a><br>
<h4>[Fig3. Crosstalk assay]<br>
<h4>[Fig3. Crosstalk assay]<br>
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This graph shows that Plux/tet hybrid promoter can suppress the crosstalk.
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<a href=""><img src="https://static.igem.org/mediawiki/2013/4/41/Titech2013_home_M13phage-plaque.png" width="200"></a><br>
<h4>[Fig4. M13 phage plaque]<br>
<h4>[Fig4. M13 phage plaque]<br>
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We confirmed inducible M13 release by plaque assay.
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<i>E. ninja</i> releases <b>M13 phage</b> as shuriken, when <i>E. ninja</i> is on attack state and receiving <i>E. samurai</i> signal. M13 phage infects <i>E. samurai</i> and causes their suicide.
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<div align="right"><a href="">(go to Shuriken page)</a></div>
<div align="right"><a href="">(go to Shuriken page)</a></div>
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We have also made second-life of ninja. After ninja retires his ninja job, he starts <b>farming</b> in the village. We aim to create farmer <i>E. coli</i>, which can synthesize plant hormone depending on soil environment.
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<div align="right"><a href="">(go to Farming page)</a></div>
<div align="right"><a href="">(go to Farming page)</a></div>
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<a href=""><img src="https://static.igem.org/mediawiki/2013/9/9b/Titech2013_home_plant-bioassay.png" width="200"></a><br>
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<h4>[Fig5. Bioassay of plant]<br>
<h4>[Fig5. Bioassay of plant]<br>
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We learned methods for quantitative analysis for cytokinin, a plant hormone, through bioassay of cucumber seed sprouts.
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<div align="right"><a href="">(see more)</a></div>
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Revision as of 12:38, 23 September 2013

Abstract

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[Fig1. Human Practice experiment]
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[Fig2. Human Practice enquete]
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x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x

x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x


[Fig3. Crosstalk assay]
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[Fig4. M13 phage plaque]
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x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x

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[Fig5. Bioassay of plant]
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