Revision as of 04:49, 24 September 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit to Profile Aromatics in Environment

Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge. Noting the power of biosensors to enable quick and convenient testing, Peking iGEM have developed a novel biosensor toolkit to profile aromatics in environment. Transcriptional regulators that each senses a specific class of aromatics were first bioinformatically determined; they were then utilized to build a comprehensive set of biosensor circuits. These biosensors proved to be high-performance.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable chemicals into detectable aromatics, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We have a date in the database

Auto Sensor Mining

Light of glory shall shed
upon those who see

Biosensors

They will always be there
waiting for our arrival

Plug-ins

My heart beats only for the special you

Band-pass Filter

Though soundless the beatings of wings
do feel the pleasant breeze

Human Practice

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-             <p id="AbstractContent">There are numerous classes of prokaryotes lives in the aromatics-rich environment. Part of them possess aromatic degradation pathways, and also transcriptional factors to monitor the environmental aromatics, and control degradation pathway to balance the degradation capacity. Peking iGEM team mainly focused on the various transcriptional factors, which can respond to various aromatic inducers. We characterized these biosensors and improve their performance by using well-characterized biobricks. We expanded the detection range by coupling enzymes in upper pathways and biosensors to degrade aromatics to detectable compounds. For quantitative detection, we construct the band pass filter to detect a certain range of concentration. The appliance of microfluidics may construct a concentration gradient, and a certain concentration’s response can reflect the original concentration of environmental samples. With the application of this device, we can achieve easy and quantitative detection of environmental aromatics, and at the same time, remarkably enrich the library of inducible biosensors.
+              <p id="AbstractContent">Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in the environment, however, remains a substantial challenge. Noting the power of biosensors to enable quick and convenient testing, Peking iGEM have developed a novel biosensor toolkit to profile aromatics in environment. Transcriptional regulators that each senses a specific class of aromatics were first bioinformatically determined; they were then utilized to build a comprehensive set of biosensor circuits. These biosensors proved to be high-performance.
+<br/><br/>
+Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable chemicals into detectable aromatics, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.
 </p>
          </div>

Team:Peking

From 2013.igem.org