Team:UNITN-Trento/Parts
From 2013.igem.org
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<td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> | <td class="code"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1065310">BBa_K1065310</a></td> | ||
- | <td class="name"> | + | <td class="name">Complete blue-light sensing circuit with an inverter</td> |
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- | + | Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. | |
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Revision as of 15:13, 24 September 2013
In this page you can find the fruits of our summer’s efforts. The number of bananas quantifies how much we like each part:
: This is an intermediate part and it has not been charachterized (METTERE LA BANANA VERDE!!).
: the part does not work, due to a mistake of some kind, or we do not know if it works and more tests must be performed.
: the part was charachterized but doesn't work always as espected.
: the part works as expected and has been characterized.
♫ ♪ “One banana, two bananas, three bananas, four. Four bananas make a bunch and so do many more.” ♪ ♫
[cit. The Banana Splits – The Tra La La Song]
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BBa_K1065000 | EFE |
Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. |
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BBa_K1065002 | EFE with double-terminator |
EFE followed by the double terminator BBa_B0015. |
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BBa_K1065001 | EFE arabinose-regulated |
This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. |
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BBa_K1065100 | BSMT1 |
This part encodes BSMT1, an enzyme that methylates salycilic acid to obtain methyl salycilate. It is the original part from the 'Eau de coli' MIT iGEM 2006 project, re-submitted (and resurrected) by us in pSB1C3. |
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BBa_K1065103 | PchBA |
PchA is the enzyme responsible for the formation of isochorismate from chorismate. The next step is performed by the PchB enzyme, which converts isochorismate in salicylic acid. |
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BBa_K1065104 | SAM synthetase |
This part encodes the SAM (S-AdoMet) synthetase gene, which we friendly call "uncle SAM". SAM is a methyl donor required to produce MeSA and other methylated molecules. |
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BBa_K1065101 | BSMT1 arabinose-regulated |
A methyltransferase (BSMT1) regulated by the araCpBAD promoter. |
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BBa_K1065105 | SAM synthetase under pLac promoter |
This is SAM synthetase (uncle SAM) regulated by the constitutive Lac promoter. |
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BBa_K1065102 | PchBA under pLac and BSMT1 arabinose-regulated |
The wintergreen device: PchBA under a mutant Lac promoter (BBa_K1065107) followed by BSMT1 under the araCpBAD promoter. |
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BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
An improved version of the wintergreen device: BBa_K1065102 with SAM synthetase to boost production. |
BBa_K1065306 | J23100+Yf1+FixJ | |
Yf1 (photosensor) and FixJ (response regulator) under the Anderson promoter (BBa_J23100). |
BBa_K1065303 | Yf1+FixJ+pFixK2 | |
The starting point to build a blue-light sensing circuit: the photosensor (Yf1) and the response regulator (FixJ) with its promoter (pFixK2). This part was build merging BBa_K592016 and BBa_K592006. |
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BBa_K1065302 | Blue light circuit to produce amilGFP |
A device to charachterized our improved part BBa_K1065305 controlled by pLac promoter. |
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BBa_K1065305 | BBa_K952003 improved |
No rbs, no party!!!!We improved BBa_K952003 by mutagenesis to add the missing RBS, now the part works. |
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BBa_K1065309 | Blue light ethylene producing device |
Complete blue-light sensing circuit without inverter, producing both amilGFP and EFE. |
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BBa_K1065310 | Complete blue-light sensing circuit with an inverter |
Yf1 and FixJ are regulated by the Anderson promoter (BBa_J23100). pLambda regulates the expression of the reporter gene amilCP. When exposed to blue-light, amilCP is synthesized, when the blue-light is not present, amilCP is not produced. |
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BBa_K1065311 | Complete blue-light sensing device with inverter, amilCP and EFE |
Our complete circuit composed by:J23100, Yf1 and FixJ, pFixK2, CI, pLambda, amilCP and EFE. |
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BBa_K1065201 | pXyl+GFP |
A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). |
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BBa_K1065203 | Efe+Bba_B0015 in pSpac. |
EFE in pSpac vector, specific for B. subtilis. |
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BBa_K1065204 | pXyl+EFE |
EFE in pXyl vector, specific for B. subtilis. |
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