Team:USTC CHINA/Notebook/Protocols

From 2013.igem.org

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<h>Gene Clone</h>
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<div class="protocol-col-sub">
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<h1>Gel Extraction</h1>
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<h1>DNA digestion</h1>
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<h2>Single Digestion</h2>
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<h2>Double Digestion</h2>
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<h1>Colony PCR</h1>
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<h1>PCR</h1>
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<h1>Ligation</h1>
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<h1>Plasmid mini-prep</h1></div>
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<div class="protocol-col-sub">
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<h1>Transformation</h1>
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<h2.The transformation of E.coli</h2>
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<h2>The transformation of Bacillus subtilis</h2>
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<h1>Overlap PCR</h1>
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<h1>Extracting plasmids</h1>
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<h2>Extracting plasmids from gram-positive bacterium</h2>
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<h2>Extracting plasmids from gram-negative bacterium<h2></div>
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</div>
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<div class="protocol-col-2">
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<h>Expression of proteins</h>
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<h1>Expression of proteins in Bacillus subtilis</h1>
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<h1>Expression of proteins in Escherichia coli</h1>
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<h1>Concentrating proteins</h1>
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</div>
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<div class="protocol-col-2">
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<h>Transdermal experiments</h>
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</div>
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<div class="protocol-col-2">
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<h>Immunity&Tests</h>
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<h1>ELISA</h1>
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<h1>Sample analysis</h1>
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</div>
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<div class="protocol-col-2">
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<h>Medium</h>
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<h1>LB media</h1>
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<h1>10*S base</h1>
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<h1>HS media</h1>
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<h1>LS media</h1>
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<h1>2*YT media</h1>
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<div class="protocol-col-2">
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<h>Measuremen</h>
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<h1>Constitutive promoter measurements</h1>
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<h1>plac promoter response to IPTG or Glucose</h1>
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<div class="protocol-col-2"></div><div class="protocol-col-2"></div>
 
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Revision as of 11:17, 25 September 2013

Gene Clone

Gel Extraction

DNA digestion

Single Digestion

Double Digestion

Colony PCR

PCR

Ligation

Plasmid mini-prep

Transformation

The transformation of Bacillus subtilis

Overlap PCR

Extracting plasmids

Extracting plasmids from gram-positive bacterium

Extracting plasmids from gram-negative bacterium

Expression of proteins

Expression of proteins in Bacillus subtilis

Expression of proteins in Escherichia coli

Concentrating proteins

Transdermal experiments
Immunity&Tests

ELISA

Sample analysis

Medium

LB media

10*S base

HS media

LS media

2*YT media

Measuremen

Constitutive promoter measurements

plac promoter response to IPTG or Glucose

notebook

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"