Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis

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Revision as of 14:12, 25 September 2013

Expression of proteins in Bacillus subtilis

Induction with IPTG - grow appropriate B. subtilis strain overnight in fresh 2xYT medium - inoculate into fresh 2xYT medium to an OD600 of 0.15 - when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG (CB-0487-1GAM) to one portion (t = 0) - collect samples at different time points for analysis (t = 1,…)

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