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Team:USTC CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis
From 2013.igem.org
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- grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br> | - grow appropriate B. subtilis strain overnight in fresh 2xYT medium</br> | ||
- inoculate into fresh 2xYT medium to an OD600 of 0.15</br> | - inoculate into fresh 2xYT medium to an OD600 of 0.15</br> | ||
| - | - when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM IPTG(CB-0487-1GAM) to one portion (t = 0)</br> | + | - when culture reaches OD600 0.7 – 0.8, split into 2 portions and induce with 1 mM </br> IPTG(CB-0487-1GAM) to one portion (t = 0)</br> |
- collect samples at different time points for analysis (t = 1,…)</br> | - collect samples at different time points for analysis (t = 1,…)</br> | ||
</p></div> | </p></div> | ||
Revision as of 15:01, 25 September 2013
