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Team:KIT-Kyoto/nf08123
From 2013.igem.org
(Difference between revisions)
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<h2>Protocol</h2> | <h2>Protocol</h2> | ||
| + | <p><b><font size="4">Miniprep</font></b></p> | ||
| - | < | + | <p><b><font size="4">Rapid check of the insert by colony cracking</font></b></p> |
| - | <p><b><font size="4"> | + | |
| + | |||
| + | <p><b><font size="4">DNA purification and precipitation</font></b></p> | ||
| + | |||
| + | |||
| + | <p><b><font size="4">PCR</font></b></p> | ||
| + | |||
| + | |||
| + | <p><b><font size="4">SDS polyacrylamide gel electrophoresis (SDS-PAGE)</font></b></p> | ||
| + | |||
| + | |||
| + | <p><b><font size="4">Isolation and purification of DNA bands</font></b></p> | ||
| + | |||
| + | |||
| + | <p><b><font size="4">Ligation</font></b></p> | ||
| + | |||
| + | |||
| + | <p><b><font size="4">Transformation</font></b></p> | ||
Revision as of 02:48, 26 September 2013
Protocol
Miniprep
Rapid check of the insert by colony cracking
DNA purification and precipitation
PCR
SDS polyacrylamide gel electrophoresis (SDS-PAGE)
Isolation and purification of DNA bands
Ligation
Transformation
