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Team:HIT-Harbin/Parts
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<h5>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</h5> | <h5>CODON OPTIMIZATION FOR HRPS AND HRPR GENE</h5> | ||
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| - | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2013/f/f8/Hrps_sequence_cai_before_optimization.png"/> |
<p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p> | <p>Figure1: Codon Adaptation of hrpS gene in E.coli before optimization</p> | ||
</div> | </div> | ||
<p></p>CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17% | <p></p>CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17% | ||
<div align="center"> | <div align="center"> | ||
| - | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2013/d/d3/Hrps_sequence_cai_after_optimization.png"/> |
<p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p> | <p>Figure2: Codon Adaptation of hrpS gene in E.coli after optimization</p> | ||
</div> | </div> | ||
<p></p>CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60% | <p></p>CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60% | ||
<div align="center"> | <div align="center"> | ||
| - | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2013/a/a8/Hrpr_sequence_cai_before_optimization.png"/> |
<p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p> | <p>Figure3: Codon Adaptation of hrpR gene in E.coli before optimization</p> | ||
</div> | </div> | ||
<p></p>CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70 | <p></p>CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70 | ||
<div align="center"> | <div align="center"> | ||
| - | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/2013/8/8b/Hrpr_sequence_cai_after_optimization.png"/> |
<p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p> | <p>Figure4: Codon Adaptation of hrpR gene in E.coli after optimization</p> | ||
</div> | </div> | ||
Revision as of 00:17, 27 September 2013
Parts
Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote
CODON OPTIMIZATION FOR HRPS AND HRPR GENE
Figure1: Codon Adaptation of hrpS gene in E.coli before optimization
Figure2: Codon Adaptation of hrpS gene in E.coli after optimization
Figure3: Codon Adaptation of hrpR gene in E.coli before optimization
Figure4: Codon Adaptation of hrpR gene in E.coli after optimization
reference
[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33<groupparts>iGEM013 HIT-Harbin</groupparts>
