Team:Hong Kong HKUST/Project/module1
From 2013.igem.org
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<h3>Cell Culture</h3> | <h3>Cell Culture</h3> | ||
- | HepG2 and HEK293FT cells were grown in DMEM supplemented with 10% heat-inactivated FBS, | + | HepG2 and HEK293FT cells were grown in DMEM supplemented with 10% heat-inactivated FBS, 50 μg/mL penicillin and 50 μg/mL streptomycin. The cells were incubated at 37℃ in a humidified atmosphere containing 5% CO2. Cells were transfected in petri dishes and multi-well plates with Lipofectamine 2000 (Invitrogen; Carlsbard, CA) transfection reagent according to the manufacturer’s protocols. GFP signals were observed under fluorescent microscope or under confocal microscope, if necessary. |
</div> | </div> | ||
</div> | </div> |
Revision as of 19:34, 26 September 2013
![](https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png)
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FA Quantification & Cell Viability
- Cell Line
- Cell Culture
- Cell Viability
- Fatty Acid Quantification
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Modules
- FA Quantification & Cell Viability
- FA Sensing Mechanism
- Protein Trafficking
- Glyoxylate Shunt