Team:Goettingen/Project

From 2013.igem.org

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===Target: c-di-AMP===
===Target: c-di-AMP===
<p>Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have marked a major victory of mankind in the battle against infectious diseases. However, after 90 years, the antibiotics are now losing their old time glory: Bacteria acquire resistance against antibiotics and become unbridled.</p>
<p>Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have marked a major victory of mankind in the battle against infectious diseases. However, after 90 years, the antibiotics are now losing their old time glory: Bacteria acquire resistance against antibiotics and become unbridled.</p>
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<p>We must control the use of antibiotics, meanwhile, we need new antibiotics, which can suffiently eliminate the invaders without hurting the "good" bacteria. Therefore, c-di-AMP, an important, recently discovered signaling molecule in gram-positive bacteria, has come to our sight.</p>
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<p>We should have better control over the use of antibiotics, meanwhile, we need to develop new ones, which can sufficiently eliminate the invaders without hurting the "good" bacteria. Therefore, c-di-AMP, an important, recently discovered signaling molecule in gram-positive bacteria, has come to our sight.</p>
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<p>Our project is to build a screening system targeting c-di-AMP, which could be applied in novel-drug screening. With this system, the level of c-di-AMP in the cell can be visualised and measured. In the moment we are still wokring hard in the lab. To see our progress, please visit [[Team:Goettingen/NoteBook|Our Lab Book]].</p>
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<p>Our project is aimed at finding a way to fight against multi-resistant bacteria by targeting c-di-AMP. We made three different approaches, each approach was accomplished by each subteam, for detailed information, please click the icon on the right panel</p>
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<p>We seperated our team into three subteams, the Array team, DAC team and the Reporter team. To know about the subteams, please click the icon on the right side.</p>
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<p style="margin-left:20px; " class="goe-rt">first, we have built two screening systems, which could be applied in novel-drug screening. With this system, the level of c-di-AMP in the cell can be visualized and measured</p>
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<p>Our team conducted our work on the North Campus of University Goettingen, in the Microbiology department. All work was done in our S1 level lab. We follow strickly the safety S1 instructions. To know more concerning safety issues, please [[Team:Goettingen/Safety|visit our safety page]].
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<p style="margin-left:20px; " class="goe-dac">We also looked into the diadenylate cyclase(DAC), which can be a great novel target for new antibiotics. We have expressed the truncated DAC(the catalytical domain) from <i>Listeria monocytogenes</i> in <i>E.coli</i>, characterized and  crystallized it and finally got a STRUCTURE. </p>
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<p style="margin-left:20px" class="goe-array">We searched for the genes effect by the level of c-di-AMP and we found <i>ydaO</i>.We identified the Ribo-Switch upstream <i>ydaO</i> and used it directly in our screening system.</p>
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<p>After over 3 month of work, we have finally wrapped up. To know more details about our lab work, please visit [[Team:Goettingen/NoteBook|Our Lab Book]].</p>
 +
<p>We conducted all our work on the North Campus of University Goettingen, in the Microbiology department. All work was done in our S1 level lab. We follow strickly the safety S1 instructions. To know more about safety issues, please [[Team:Goettingen/Safety|visit our safety page]].
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Revision as of 18:59, 27 September 2013





The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria


Target: c-di-AMP

Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have marked a major victory of mankind in the battle against infectious diseases. However, after 90 years, the antibiotics are now losing their old time glory: Bacteria acquire resistance against antibiotics and become unbridled.

We should have better control over the use of antibiotics, meanwhile, we need to develop new ones, which can sufficiently eliminate the invaders without hurting the "good" bacteria. Therefore, c-di-AMP, an important, recently discovered signaling molecule in gram-positive bacteria, has come to our sight.

Our project is aimed at finding a way to fight against multi-resistant bacteria by targeting c-di-AMP. We made three different approaches, each approach was accomplished by each subteam, for detailed information, please click the icon on the right panel

first, we have built two screening systems, which could be applied in novel-drug screening. With this system, the level of c-di-AMP in the cell can be visualized and measured

We also looked into the diadenylate cyclase(DAC), which can be a great novel target for new antibiotics. We have expressed the truncated DAC(the catalytical domain) from Listeria monocytogenes in E.coli, characterized and crystallized it and finally got a STRUCTURE.

We searched for the genes effect by the level of c-di-AMP and we found ydaO.We identified the Ribo-Switch upstream ydaO and used it directly in our screening system.

After over 3 month of work, we have finally wrapped up. To know more details about our lab work, please visit Our Lab Book.

We conducted all our work on the North Campus of University Goettingen, in the Microbiology department. All work was done in our S1 level lab. We follow strickly the safety S1 instructions. To know more about safety issues, please visit our safety page.


Overview

Array Team

DAC Team

Reporter Team

Parts

Collaboration

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