Team:USTC CHINA/Project/Results
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- | <div class="bassic-bar"><p>① E.coli BL21 proved that the standard transdermal locus does work with GFP.</p | + | <div class="bassic-bar"> |
+ | <h1>Results</h1> | ||
+ | <p>① E.coli BL21 proved that the standard transdermal locus does work with GFP.</p> | ||
<img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="160"/> | <img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="160"/> | ||
<p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b,which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP, induced by IPTG. </p> | <p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b,which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP, induced by IPTG. </p> | ||
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<p>The molecule weight and key marker data of fusion protein TD1-GFP</p> | <p>The molecule weight and key marker data of fusion protein TD1-GFP</p> | ||
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- | <img src="https://static.igem.org/mediawiki/igem.org/2/20/2013ustc-china_ygt%28GFP%29.png"> | + | <img src="https://static.igem.org/mediawiki/igem.org/2/20/2013ustc-china_ygt%28GFP%29.png" width="290" height="290"/> |
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- | <img src="https://static.igem.org/mediawiki/igem.org/f/f9/2013ustc-china_blank.png"> | + | <img src="https://static.igem.org/mediawiki/igem.org/f/f9/2013ustc-china_blank.png width="290" height="290"/> |
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Revision as of 20:53, 26 September 2013