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Team:MSOE Milwaukee/Week4
From 2013.igem.org
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<H1 align = left>Monday</H1> | <H1 align = left>Monday</H1> | ||
| - | <p>As a team, we discussed different options for plasmids and decided to keep researching plasmids that past iGEM teams have used that were successful. We talked about needing 2 separate plasmids with two different antibiotic resistance genes because we need to distinguish between the two in the | + | <p>As a team, we discussed different options for plasmids and decided to keep researching plasmids that past iGEM teams have used that were successful. We talked about needing 2 separate plasmids with two different antibiotic resistance genes because we need to distinguish between the two in the ''E.coli''. Also, we decided on using the DE3 strain of ''E.coli'', and we already have it here at MSOE, so it will be easy to obtain. Our wiki was also updated, and we hope to keep it updated daily. </p><br> |
<H1 align = left>Tuesday</H1> | <H1 align = left>Tuesday</H1> | ||
Revision as of 18:56, 25 June 2013
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Monday
As a team, we discussed different options for plasmids and decided to keep researching plasmids that past iGEM teams have used that were successful. We talked about needing 2 separate plasmids with two different antibiotic resistance genes because we need to distinguish between the two in the ''E.coli''. Also, we decided on using the DE3 strain of ''E.coli'', and we already have it here at MSOE, so it will be easy to obtain. Our wiki was also updated, and we hope to keep it updated daily.
Tuesday
In small groups, we researched more about plasmids and found one very promising low copy plasmid. pSB3K3 has a kanamycin antibiotic resistance marker, so whichever second plasmid we choose will need to have a different marker. It is available in our kit too, which was a great find. We also began talking about ideas for our human practices portion of our project and the safety questions.
Wednesday
Thursday
Friday
