Team:Peking/HumanPractice/ModeliGEM

From 2013.igem.org

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<B><I>In September 7th, the first Model iGEM was hosted by Peking iGEM. Focusing on inter-team communication, preparing for iGEM jamboree but not for prize, Model iGEM is a competition without competitiveness. </I></B>
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In order to test if our toolkit works practically, we took water samples from our beloved Weiming Lake. Is she pure as she appears? Or is she polluted by aromatic compounds?
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Weiming Lake is a famous scenic spot in Peking University. In Chinese, "Weiming" means "without being named". Because many people in history tried to name it by capturing its essence, but all failed, so a famous scholar gave the lake this name.
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<img id="FigurePic1" src="https://static.igem.org/mediawiki/igem.org/c/cf/Peking2013_Weiminglake.jpg"/>
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We believe, that interactions bring great insights. And we all think it’s a really unique, fantastic experience. We hope other iGEM teams are inspired to do the same, and Model iGEM will be continued to host in the years after.
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</br>Located in the center of the campus, the lake was once the royal garden in Qing Dynasty. Today it is still regarded as a sacred lake by many visitors. On the hill of the southern part of the Weiming Lake, there is a pagoda named Boya. It is an imitation of ancient tower. The combination of the lake and the tower forms a distinctive landscape. Students in Peking University can be seen reading and studying beside the lake.</br></br>
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Thus，testing water sample from this lake is meaningful for us.
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For several team members, it will be their first time to attend iGEM Asian Jamboree in this October. Model iGEM could be seen as a “drill” in advance, from presentation to poster. We want the competition to work like the Asian Jamboree as much as possible, in the meanwhile, promote the communication between teams.<br/>
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However, it’s inappropriate and unnecessary to award any prize, for few teams have adequate time to get ready in early September.
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<B>Plans</B><br/>
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Opening Ceremony<br/>
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8:40 a.m.       Opening Remarks by Dr. Chunbo Lou<br/>
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Presentation Competition<br/>
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9:15- 9:45      Pre of Peking<br/>
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9:45- 10:15    Pre of BIT<br/>
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10:15-10:45    Pre of Fudan<br/>
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10:45-11:00    <I>Tea break</I><br/>
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11:00-11:30    Pre of NJU-China<br/>
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11:30-12:00    Pre of Tianjin<br/>
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12:00-12:30    Pre of OUC-China<br/><br/>
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Each team has 20 minutes for presentation, 5 minutes for Q&A and another 5 minutes for team-switching during Presentation Competition.
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Poster Competition<br/>
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12:30-14:00    Lunch at posters<br/><br/>
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Closing Ceremony<br/>
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14:30-15:30  Closing remarks by Haoqian Zhang<br/>
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            Team communication and group photo-taking<br/><br/>
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<B>Snap shot</B><br/>
 
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Concentrating on ideas from different teams, we really enjoyed our time. Strengths and weaknesses, we sometimes saw in ourselves and from others. Wonderful questions from audiences and judges pointed out how to improve and present our projects in the future, so does advice.
 
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<img id="FigurePic2" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_Testingwater.jpg"/>
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Having experienced a model competition, we owned a chance to view our project as an outsider. And we believe that, we will be much more at ease in the presentation of Asian jamboree.  
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Water from Weiming Lake in Peking University was sampled at 15:30 and 19:30, sterilized by 0.22 um filter. LB solution was prepared by using each of the water samples. Antibiotics were added into two tubes containing the two water samples to prepare 50 ml engineered bacteria’s medium. After 12 hours cultivation according to the inducing process, OD 600 and F515 were tested. Then, aromatic compounds was calculated by dose-effect curve.
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As for lunchtime, we enjoyed the tasty pizza and our talk. We shared funny stories in this summer and exchanged ideas about different projects as well as teams. Walking around, we viewed posters from each team, and discussed curves, data, even details about materials and test conditions.
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The aromatic compounds concentration was parallelly measured three times by microplate reader. The induction ratios are as follow. According to the table, the water samples didn’t induce the manipulated bacteria obviously and each kind of aromatic compounds was below the detection limit.
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According to the experiment, Weiming Lake’s water sample didn’t induce manipulated bacteria obviously. It can be drawn that benzoic acid, aminosalicylic acid and phenylog derivatives concentration of Weiming Lake are all under the water pollution standard of our country (GB8978-1996).
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The conclusion are drawn as follow, water from Weiming Lake is not natural water, but running water from taps, which was purified by complex procedures. It is anticipatable that the water from Weiming Lake is up to standard and there is no aromatic compounds within.
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<img id="FigurePic3" src="https://static.igem.org/mediawiki/igem.org/c/c6/Peking2013_Testwaterresult.jpg"/>
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Eventually, closing remarks approached. Tired but joyful, we will never forget this rewarding model jamboree.
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</br>To see our test protocols,please <ahref="https://2012.igem.org/Team:Peking/Team/Notebook/Protols">click here</a>.
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Revision as of 15:49, 27 September 2013

Practical Analysis

In order to test if our toolkit works practically, we took water samples from our beloved Weiming Lake. Is she pure as she appears? Or is she polluted by aromatic compounds?

Weiming Lake is a famous scenic spot in Peking University. In Chinese, "Weiming" means "without being named". Because many people in history tried to name it by capturing its essence, but all failed, so a famous scholar gave the lake this name.


Located in the center of the campus, the lake was once the royal garden in Qing Dynasty. Today it is still regarded as a sacred lake by many visitors. On the hill of the southern part of the Weiming Lake, there is a pagoda named Boya. It is an imitation of ancient tower. The combination of the lake and the tower forms a distinctive landscape. Students in Peking University can be seen reading and studying beside the lake.

Thus，testing water sample from this lake is meaningful for us.

Water from Weiming Lake in Peking University was sampled at 15:30 and 19:30, sterilized by 0.22 um filter. LB solution was prepared by using each of the water samples. Antibiotics were added into two tubes containing the two water samples to prepare 50 ml engineered bacteria’s medium. After 12 hours cultivation according to the inducing process, OD 600 and F515 were tested. Then, aromatic compounds was calculated by dose-effect curve.

The aromatic compounds concentration was parallelly measured three times by microplate reader. The induction ratios are as follow. According to the table, the water samples didn’t induce the manipulated bacteria obviously and each kind of aromatic compounds was below the detection limit.

According to the experiment, Weiming Lake’s water sample didn’t induce manipulated bacteria obviously. It can be drawn that benzoic acid, aminosalicylic acid and phenylog derivatives concentration of Weiming Lake are all under the water pollution standard of our country (GB8978-1996).

The conclusion are drawn as follow, water from Weiming Lake is not natural water, but running water from taps, which was purified by complex procedures. It is anticipatable that the water from Weiming Lake is up to standard and there is no aromatic compounds within.


To see our test protocols,please click here.

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