"
Team:HokkaidoU Japan/Notebook/RBS SD2,4,6-Reconstruction
From 2013.igem.org
(Difference between revisions)
(Created page with "{{Team:HokkaidoU_Japan/header_Maestro}} <html> <div id="common-header-bottom-background"> <div class="wrapper"> <h1 id="common-header-title">Maestro E.coli</h1> ...") |
|||
| Line 12: | Line 12: | ||
<div id="hokkaidou-contents"> | <div id="hokkaidou-contents"> | ||
<!-- end header / begin contents --> | <!-- end header / begin contents --> | ||
| - | <h2></h2> | + | <h2>SD2,4,6 Reconstruction</h2> |
<div class="workflow-container"> | <div class="workflow-container"> | ||
<ul class="steps"> | <ul class="steps"> | ||
| Line 19: | Line 19: | ||
</li> | </li> | ||
| - | + | <li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jun 15</time> </li> </ul> <div class="step-recipe"> R0040-SD8-LacZα-B0015 100up SD(6/4/2) mutation revers </div> </div> </div> </li> | |
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Gel extraction</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jun 15</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jun 15</time> </li> </ul> <div class="step-recipe"> Cut R0040-SD(6/4/2) with EcoRI and SpeI (10×H buffer) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ethanol precipitation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jun 16</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2) </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Jun 16</time> </li> </ul> <div class="step-recipe"> Ligate R0040-SD(6/4/2) with LacZα-B0015 </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Jun 17</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2)-LacZα-B0015<br> each of 5µL DNA to DH5α </div> </div> </div> </li> | ||
| + | <li class="step"> <div class="step-tile"> <h2 class="step-title">Sequencing</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jun 22</time> </li> </ul> <div class="step-recipe"> R0040-SD(6/4/2)-LacZα-B0015 </div> </div> </div> </li> | ||
<li class="step complete"> | <li class="step complete"> | ||
<div class="step-tile"><h2>Complete!</h2></div> | <div class="step-tile"><h2>Complete!</h2></div> | ||
Latest revision as of 16:38, 27 September 2013
Maestro E.coli
Notebook
SD2,4,6 Reconstruction
-
Start
-
PCR
R0040-SD8-LacZα-B0015 100up SD(6/4/2) mutation revers -
Gel extraction
R0040-SD(6/4/2) -
Digestion
Cut R0040-SD(6/4/2) with EcoRI and SpeI (10×H buffer) -
Ethanol precipitation
R0040-SD(6/4/2) -
Ligation
Ligate R0040-SD(6/4/2) with LacZα-B0015 -
Transformation
R0040-SD(6/4/2)-LacZα-B0015
each of 5µL DNA to DH5α -
Sequencing
R0040-SD(6/4/2)-LacZα-B0015 -
Complete!
