Team:WHU-China/modelingintro

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Revision as of 02:27, 29 October 2013

Modeling introduction
The primary aim of our model is to enable people to design their own multi-stage promoter. It consist of 3 part. The first part is the overall user guideline of Multi-stage promoter design. The other two parts are two models that tackle with specific question.

If you want to design your own multi-stage promoter, start from here(here).

If you want to know more about tandem promoter and design your own tandem promoter, please check our TP model (TP model). The TP model aims at predicting the expression level of a tandem-repeat promoter system. With the help of this model, one can employ limited expression element to reach a desired expression level in any species, especially in non-model organisms that have few known expression element.

If you want to know more about Cas9 off-target behavior and choose your Cas9 target sequence wisely, please check our Cas9Off model (Cas9Off model). It takes a nucleic acid thermodynamics approach and analyzing all off-target studies published. Hope that it will be useful for your research!

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 <strong><h1 style="font-size:30px;color:green;">Modeling introduction</h1></strong>
-We've constructed two models.</br></br>
+The primary aim of our model is to enable people to design their own multi-stage promoter. It consist of 3 part. The first part is the overall user guideline of Multi-stage promoter design. The other two parts are two models that tackle with specific question.
+</br></br>
-The first one aims at predicting the expression level of a tandem promoter system. This model takes into consideration of the type of sub-promoters, the order of sub-promoters and the number of sub-promoters. With the help of this model, one can employ limited expression element to reach a desired expression level in any species, especially in non-model organisms that have few known expression element. </br></br>
+If you want to design your own multi-stage promoter, start from here(<a href="https://2013.igem.org/Team:WHU-China/modelguide">here</a>).</br></br>
-The second model aims at bringing in some more insight about the off-target behavior of Cas9 by taking a new approach and analyzing all off-target studies published. In the analysis of Cas9 binding, it revealed the potentiality of nucleic acid thermodynamic model in Cas9 off-target predicting, as well as its limits. It suggested that the gRNA-Cas9 will tolerate mismatches more generously when the gRNA-DNA binding energy is too high or too low. After the dCas9 off-target rate analyzed, kinetic of Cas9 cleaving is derived. And the following concentration-time function of the cleaved product suggested that expression time of Cas9 is as important as the concentration of Cas9.
+If you want to know more about tandem promoter and design your own tandem promoter, please check our TP model (<a href="https://2013.igem.org/Team:WHU-China/modelingtp">TP model</a>). The TP model aims at predicting the expression level of a tandem-repeat promoter system. With the help of this model, one can employ limited expression element to reach a desired expression level in any species, especially in non-model organisms that have few known expression element.
+</br></br>
+If you want to know more about Cas9 off-target behavior and choose your Cas9 target sequence wisely, please check our Cas9Off model (<a href="https://2013.igem.org/Team:WHU-China/modelingCas9">Cas9Off model</a>). It takes a nucleic acid thermodynamics approach and analyzing all off-target studies published. Hope that it will be useful for your research!
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