Team:SUSTC-Shenzhen-A/Project

Abstract

There are many applications of the game theory in some aspects of our life. Each individual has two kinds of choices--to betray or stay silent, and the choice you make would determine your fate. To betray the other side, you may risk being revenged. While staying silent, companion's betrayal may hurt you deeply. As for our project, we work out a new way to imitate the game theory by constructing a community of two E. Coli bacteria. Here we use the growth rate of each species to represent its fate. The effect of one's silent or betrayal on the other species' fate is acted through intercellular signal molecules of two quorum sensing systems. Each signal molecule regulates the expression of toxic genes in the other species and reduces its growth rate. We characterize the consequence of each strategy by quantitatively measure the growth rates of each species in the community.

Background

We find that most of the researches in synthetic biology aim to study the interaction between parts of a single system. But few focus on the interaction between systems with multi-elements. However, in the real world, there are complex competition and cooperation among the various creatures. And they can work together to achieve a certain function, for example, different kinds of yeast in brewing beer. There is no lack of the ideas of game theory in these processes. So we chose a classical method—prisoner's dilemma as a model to design our project.

Our project can simulate the interaction among multiple systems in reality and the final state of them. Through a well-known issue of game theory, we can give synthetic biology and iGem greater publicity from many perspectives. Furthermore, after a slight modification, the project can provide us with an interesting solution to security.

Project Details

our design

Parts:

Communication: quorum sensing

LuxI produces 6HSL, 6HSL crosses membrane, 6HSL binds to LuxR, LuxR activates promoter Plux

LasI produces 12HSL, 12HSL crosses membrane, 12HSL binds to LasR, LasR activates promoter Plas

Poissonness genes:

tetA: import Nickel into cell, which kills cell

Zeo-r: Zeocin damages DNA and kills cell, Zeo-r binds and neutralizes Zeocin

Truth Table

Tuning gene expression: repressor and inducer

Cell A: Constant expressions:

LuxR(with C6HSL, activate pLux, express Zeo-r): promoter J23100 and rbs B0034

Pcon: promoter J23100 to J23107 and rbs B0034

YFP (yellow fluorescent protein labeled cell A): promoter pCon, rbs

Adjustable expressions:

Zeo-r, adjusted by C6HSL, produced by LuxI from cell B, depends on cell B density

LasI: adjusted by Pcon, LasI produces C12HSL, controls cell B

tetA: adjusted by Pcon

Zeocin, Nickel concentration are controlled by you

C12SHL concentration: controlled by cell A concentration

Nickel either kills cell or slows cell growth, tetA increases sensitivity of cell B to Nickel

zeocin either kills cell or slows cell growth,Zeo-r decreases sensitivity of cell B to zeocin

-asv: amino acid sequence that increase response time of LuxI, tetA and Zeo-r to regulation

pMB1 and p15A: control plasmid replication in E coli

Kan and Cam(r): with antibiotics Kanamycin and chloramphenicol, prevent lost of plasmids

Cell B: Constant expressions:

LasR (with C12HSL, activate pLux, express Zeo-r): promoter J23100 to J23107 and rbs B0034

RFP (red fluorescent protein labeled cell B): promoter pCon, rbs

Adjustable expressions:

Zeo-r, adjusted by C12HSL, produced by LasI from cell A, depends on cell A density and Pcon.

LuxI: adjusted by Pcon, LuxI produces C6HSL, control cell A

tetA: adjusted by Pcon

Zeocin, Nickel concentration are controlled by you

C6HSL concentration: controlled by cell B concentration and Pcon

tetA increases sensitivity of cell B to Nickel,Nickel either kills cell or slows cell growth

Zeo-r decreases sensitivity of cell B to zeocin, zeocin either kills cell or slows cell growth

-asv: amino acid sequence that increase response time of LuxI, tetA and Zeo-r to regulation

pMB1 and p15A: control plasmid replication in E coli

Kan and Cam(r): with antibiotics Kanamycin and chloramphenicol, prevent lost of plasmids

Experiment process

Please refer to our protocol and notes for more details.

Results

project results

These pictures show us the growth curves with E coli containing our biobricks with tet and the growth rate vesus tetA level with Nickel

[http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030000 BBa_K1030000] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030001 BBa_K1030001] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030002 BBa_K1030002] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030003 BBa_K1030003] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030004 BBa_K1030004] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030005 BBa_K1030005] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030006 BBa_K1030006] [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030007 BBa_K1030007]

We use different synthetic constitutive promoter, varying from J23100 to J23107 to drive the expression of tetA, an tetracycline transporter that can also transport toxic nickel ion into cell which reduce e coli growth rate and kill cells. An asv degradation tag is added to the c-terminal of tetA. A superfolder GFP is expressed under the control of the same promoter so that we can use fluorescent plate reader, flow cytometer and microscope to monitor the expression level of tetA. They are not fused.

We generated plasmids, in which tetA and GFP are under control of 8 constitutive promoters of various intensities. There are J23100 through J23107.

We first check the growth curve of E coli containing our biobricks with various tetracyclin concentration. We grow then in Luria Broth (LB) using plate reader. The data is shown in biobricks section. With gradually increasing in tetracylcin concentration,the growth is slowing down and eventually turning negative. We fit the growth rate to the exponential growth phase of each sample and plot them against the mean GFP level, which representing the tetA level. It clearly shows the positive correlation between growth rates of E coli and the levels of tetA, an transporter than pump tetracyclin out of E coli.

We then added Nickel Chloride of various concentrations to the E coli containing the biobricks above, and growth them in Luria Broth (LB) using fluorescent plate reader. The growth curves are shown in the biobricks section. With increasing in Nickel concentration, the growth is also slowing done to negative. We then plot the growth rate against the mean GFP level. Most of the biobricks fit the negative relation between tetA and growth rate with Nickel. However, the trend is not as robust as with tet. In addition, even with lowest tetA level, there is still toxic effect with nickel, which reduced the significance of tuning growth rate via tuning tetA. We will try to find out why.

[http://parts.igem.org/wiki/index.php?title=Part:BBa_K1030008 BBa_K1030008]

What we have done are the plasmid construction and the early experiments ,because of the time limite. Here are some results of our project

We use a zeocin resistant gene and external zeocin to tune the cell growth rate. The common antibiotic resistant genes are reserved for plasmid backbones. GFPuv is used to monitor the expression of zeocin resistant gene. In addition, zeocin resistant gene allosterically inhibitor the effect of zeocin, makes it easy to be quantified and mathematically modeled.

From the data we can see that when the concentration of zeocin is less than 5000ug/ml, the number of the cells is large enough and increase slowly. But when the concentration of zeocin is more than 5000ug/ml, the cells’ number decrease rapidly. It means that when the the concentration of Zeocin is not too high, the cells can grow well. It shows us that the zeocin does harm to cells but the parts we design can resistant the effect of the zeocin so that cells can grow well. We can use this part and the external zeocin to tune the cell growth rate. It is fun and meaningful.

we also used the software from SUSTC-Shenzhen-B to help us. Here is the result:

Future work

1)We use bacteriological experiments to simulate the classical prisoner’s dilemma: the length of sentence is equivalent to the slowdown of growth.

2)Because of the symmetry of the classical situation (the sentence is same to A and B), we can choose the previous cell A and cell B. Cooperate and betray will be realized by constitive promoters of different activities.

3)Before we start the experiment, we will pick the plasmids with the different combination of constitutive promoter, transform into the E.coli.

4)Cultivate the bacteria without adding Zeo nor Nickel to OD0.2-1.0. The LuxI, LasI, tetA, Zeo-r expressed by the two kinds of bacteria are almost the same.

5)Start the experiment: mix cell A and cell B and add appropriate amount of Zeo and nickel to adjust the sentence under different situation. Observe the growth curve of bacteria. Coat the plates and count the number of yellow and red colonies. The results at different times can be recorded by Flow Cytometer; the growth curve can be recorded by ELIASA.

1)This situation is more practical. Because cell A and cell B cannot adjust strategies specifically, we adjust the strategies of cell A and cell B artificially by using different promoter combination for each step and adjust the two promoters specifically at the same time in multiple experience. It can also be simulated by computer.

2)This can be achieved by microfluidic chip controlled by computer programme.

1)As the degradation speed of 6HSL and 12HSL is high and the diffusion coefficient is limited, the influence distance of cells in agar plate is limited. So we can study the interesting phenomenon of cell A and cell B forming in the agar plate.

2)If rbs is adjusted that no HSL is delivered by cells, the cell can grow at a slow speed. Paste very dilute cell A and cell B solution in the plate, at beginning, bacteria can grow in small colony and accumulate HSL partially. At this moment, the prisoner’s dilemma is begin.

3)Because of the effect on growth and the relation to distance, the increase of diameter of different two adjacent colonies and the strategies(concentration of ara and tet) are performing the space inflatable prisoner’s dilemma.

4)Between a pair of cell A and cell B, as the communication of near-end and far-end are different, the colony cannot be roundness. The asymmetric shape is an experiment of prisoner’s dilemma and also an observation of the diversity of the situation.

5)Adjust the strategies of cell A and cell B(concentration of ara and tet), observe the effect on the shape of colonies.

Biobricks checking

results

We chose four biobricks to detect after search. They are xylose,KNO₃,glucose and 12HSL.

[http://parts.igem.org/Part:BBa_K733018:Experience BBa_K733018]: xylose

First we used the microplate to detect the fluorescence of this part, because we could not see the transition point and we were wondering what it would be look like. The data we got is not so good, for we didn’t see the transition point obviously, and also has some differences comparing to the data from HKUST. According to the detecting from HKUST, fluorescence produced in about 5% xylose is more than that in 10% xylose, however what we got is fluorescence produced in 5% xylose than that in 10% xylose.

Since we didn’t get what we really want using microplate, we tried FCM this time. In 0% xylose culture, we almost got no fluorescence produced and in 0.003% xylose culture we saw obvious fluorescence came out. There was an obvious tendency that the efficiency of the promoter increased as the xylose concentration gathered up. However, with the increase of xylose concentration, the fluorescence increased choppily. What was amazing was that the fluorescence we got was 10 times brighter than the result the HKT got, which appeared to be great.

Another interesting thing is most of the cell we detected had no fluorescence produced. Then we guess that may because the xylose molecules taken in the different cell are different, or may because the copy number in each cell is different. Maybe it has some relationship with epigenetics.

[http://parts.igem.org/Part:BBa_K774007:Experience#User_Reviews BBa_K774007]: KNO₃

Like the BBa_K733018, we were wondering where the transition point is the production of fluorescence from off to on. So we have made more concentrations increasing exponentially to foster bacteria with BBa_K774007 and tested them by microplate.The result came out that with the increasing of nitrate of potash, the fluorescence produced by single cell increases slowly at the beginning but rapidly later. What a pity that we didn't see the obvious turning point between off and on.

[http://parts.igem.org/Part:BBa_K741002:Experience#User_Reviews BBa_K741002]: glucose

Seeing BBa_K741002’s figure prepared by USTC_China, we were thinking whether we could get it more efficient in decreasing the fluorescence produced by the bacteria. Besides, we though it lacked the data under the low concentration of glucose. So we measured this part by microplate in the concentration showing below. The result came out to be very good: in about 10-2.4M glucose, the fluorescence has left only a half in one cell.With the increasing of glucose, the fluorescence/OD continuously decreases, and at about 10-1M glucose, the fluorescence/OD remains very low.

References

Click the Reference to get more information.