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Team:BIOSINT Mexico/Construction
From 2013.igem.org
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| - | :Based on the system of iGEM team Slovenia 2012, we adapted | + | :Based on the system of iGEM team Slovenia 2012, we adapted the repressor system using TAL A/KRAB. The objective is to reproduced a simplex circuit in a Lactobacillus chassis. |
| - | :The main goal of our xenobiotics biosensor is to detect organochorides in different concentration in order to activated a correct answered. When the concentration is high Gal4 DBD could add to UAS and activated linA, a protein wich degrades pesticides, and at the same time express a repressor protein | + | :The main goal of our xenobiotics biosensor is to detect organochorides in different concentration in order to activated a correct answered. |
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| + | :High concentration: When the concentration is high Gal4 DBD could add to UAS and activated linA, a protein wich degrades pesticides, and at the same time express a repressor protein which inhibits the activity of the correpressor that controled the kill switch system. | ||
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| + | :Low concentration: When the concentration decrease, there is not enought UAS activity in the linA system so the repressor activity stops and the peptides that kill the bacteria starts to be produced. | ||
===Circuit=== | ===Circuit=== | ||
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[[File:Diapositiva1.JPG|center|700px]] | [[File:Diapositiva1.JPG|center|700px]] | ||
Revision as of 03:56, 28 September 2013
SmartPro
Smartpro
Construction
Repressor switch
- Based on the system of iGEM team Slovenia 2012, we adapted the repressor system using TAL A/KRAB. The objective is to reproduced a simplex circuit in a Lactobacillus chassis.
- The main goal of our xenobiotics biosensor is to detect organochorides in different concentration in order to activated a correct answered.
- High concentration: When the concentration is high Gal4 DBD could add to UAS and activated linA, a protein wich degrades pesticides, and at the same time express a repressor protein which inhibits the activity of the correpressor that controled the kill switch system.
- Low concentration: When the concentration decrease, there is not enought UAS activity in the linA system so the repressor activity stops and the peptides that kill the bacteria starts to be produced.
Circuit
