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Exeter/10 July 2013
From 2013.igem.org
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== Morning == | == Morning == | ||
| - | - BBa_K322122 (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour. | + | - [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour. |
MiniPrep of the following Parts: | MiniPrep of the following Parts: | ||
| - | - BBa_K322122, cyan pigment | + | - [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment |
| - | - BBa_K592012, magenta pigment | + | - [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment |
| - | - BBa_K592010, yellow pigment | + | - [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment |
Plasmids extracted are now in storage at -20°C. | Plasmids extracted are now in storage at -20°C. | ||
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'''Liquid cultures of the blue light module and magenta pigment''' | '''Liquid cultures of the blue light module and magenta pigment''' | ||
| - | - BBa_K592010, yellow pigment | + | - [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment |
| - | - BBa_K592012, magenta pigment | + | - [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment |
| - | - BBa_B0015, a terminator | + | - [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator |
| - | - BBa_K592005, FixJ protein intermediate | + | - [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate |
| - | - BBa_K592004, blue light sensor | + | - [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor |
'''Retry of BBa_B0034 transformation''' | '''Retry of BBa_B0034 transformation''' | ||
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Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | ||
| - | ==(Next day, results of BBa_B0034 transformation)== | + | ==(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)== |
| - | We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies. | + | We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies. |
Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
Latest revision as of 19:53, 2 October 2013
Morning
- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.
MiniPrep of the following Parts:
- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment
- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
Plasmids extracted are now in storage at -20°C.
Afternoon
Liquid cultures of the blue light module and magenta pigment
- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment
- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment
- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator
- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate
- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor
Retry of BBa_B0034 transformation
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)
We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.
Take me back to the notebook.
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