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From 2013.igem.org

Revision as of 14:58, 10 July 2013 (view source) Cv235 (Talk | contribs) (Short description of planned liquid cultures) ← Older edit		Revision as of 11:04, 16 July 2013 (view source) Fentwistle (Talk | contribs) (→Liquid culture) Newer edit →
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-	=Liquid culture=
-	* K592010 (Yellow)	+	== Morning ==
-	* K592012 (Magenta	+
-	* B0015	+
-	* K592003 (green promoter)	+
-	* Q04510 (lambda cl)	+
-	* K592005 (FixJ)	+
-	* K592002 (CcaR)	+
-	* K592001 (CcaS)	+
-	* K592004 (YF1)	+
-	Transform B0034 again	+	MiniPrep of the following Parts:
		+
		+	- BBa_K322122, cyan pigment
		+
		+	- BBa_K592012, magenta pigment
		+
		+	- BBa_K592010, yellow pigment
		+
		+	Plasmids extracted are now in storage at -20oC.
		+
		+
		+	=Afternoon=
		+
		+	'''Liquid cultures of the blue light module and magenta pigment'''
		+
		+	- BBa_K592010, yellow pigment
		+	- BBa_K592012, magenta pigment
		+	- BBa_B0015, a terminator
		+	- BBa_K592005, FixJ protein intermediate
		+	- BBa_K592004, blue light sensor
		+
		+	'''Retry of BBa_B0034 transformation'''
		+
		+	Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

---

Revision as of 11:04, 16 July 2013

Morning

MiniPrep of the following Parts:

- BBa_K322122, cyan pigment

- BBa_K592012, magenta pigment

- BBa_K592010, yellow pigment

Plasmids extracted are now in storage at -20oC.

Afternoon

Liquid cultures of the blue light module and magenta pigment

- BBa_K592010, yellow pigment - BBa_K592012, magenta pigment - BBa_B0015, a terminator - BBa_K592005, FixJ protein intermediate - BBa_K592004, blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!