Team:TU Darmstadt/materials/Buffers

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Tris
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<li>Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol).</li>
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1x PBS
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<li>8.18 g NaCl (140 mM)</li>
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<li>0.2 g KCl (2.7 mM)</li>
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<li>1.77 g Na<sub>2</sub>HPO<sub>4</sub> (10 mM)</li>
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<li>0.24 g KH<sub>2</sub>PO<sub>4</sub> (1.8 mM)</li>
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<li>Add. 1L dH<sub>2</sub>O</li>
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<li>Adjust to pH 7.4
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Revision as of 20:13, 4 October 2013







Labbook | Materials | Protocols

Buffers

Tris

  • Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol).

1x PBS