Team:TU Darmstadt/materials/Primers

From 2013.igem.org

(Difference between revisions)

Latest revision as of 01:43, 5 October 2013

Lab book | Materials | Protocols

Primers

VR: ATTACCGCCTTTGAGTGAGC
VF2: TGCCACCTGACGTCTAAGAA

Suffix-R: TACTAGTAGCGGCCGCTGCAGGGTAA
Prefix-R: GTAGTAGAATTCGCGGCCGCTTCTAG

LO-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATGATTTGTACAGCTCGTCCATGCC
LO-pre-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTGAGCAAAGGCGAAGAAAATAACATGG

mKate-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATTAATTCAGTTTATGACCCAGGTTGCTCG
mKate-pre-ATG-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTAAGCAAAGGTGAAGAGTTAATCAAGGAGA

lssmorange ppr: CCCCCTGCAGGTTTATTATTTGTACAGCTCGTCC
lssmorange pprf: ATATGGCTAGCATGGTGAGCAAAGGCG

mkate ppr: GTCCCCCTGCAGGTTTATTAATTCAGTTTATG
mkate pprf: TACATATGGCTAGCATGGTAAGCAAAGGTG

frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag1_for: CGGCCGCTTCTAGAGACATTGATTATTTGCACGGCGT

frag2_rev: CAGTGTGATTATTAATTCAGTTTATGACCCAGGTTGCTCG
frag2_for: GGCTAGCGGAAAGAGGGGACAAATGTCAAAAATCC

frag3_rev: ATCAATGTAAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCT
frag3_for: TTAATAATCACACTGGCTCACCTTCGG

frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag4_for: ATTATTTACATTGATTATTTGCACGGCGT

frag5_rev: CGGCCGCTACTAGTATTATCATTTGTACAGCTCGTCCATGC
frag5_for: TTTTTTTGGGCTAGCGGAAAGAGGGGACAAATGATTAACCG

vector_rev: CTCTAGAAGCGGCCGCGA
vector_for: TACTAGTAGCGGCCGCTGC

Pre-PezT: GGATCCTGCAGCGGCCGCTACTAGTACTCGAGCGCTGCTGCCA
For-PezT: ATACCGAATTCGCGGCCGCTTCTAGATGGAAATCCAAGATTATACTG

@@ Line 275: / Line 275: @@
 <br>
-Suffix-R:<br>
+Suffix-R: TACTAGTAGCGGCCGCTGCAGGGTAA<br>
-Prefix-R:<br>
+Prefix-R: GTAGTAGAATTCGCGGCCGCTTCTAG<br>
 <br>
-LO-suf-R:<br>
+LO-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATGATTTGTACAGCTCGTCCATGCC<br>
-LO-pre-F:<br>
+LO-pre-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTGAGCAAAGGCGAAGAAAATAACATGG<br>
 <br>
-mKate-suf-R:<br>
+mKate-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATTAATTCAGTTTATGACCCAGGTTGCTCG<br>
-mKate-pre-ATG-F:<br>
+mKate-pre-ATG-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTAAGCAAAGGTGAAGAGTTAATCAAGGAGA<br>
 <br>
-lssmorange ppr:<br>
+lssmorange ppr: CCCCCTGCAGGTTTATTATTTGTACAGCTCGTCC<br>
-lssmorange pprf:<br>
+lssmorange pprf: ATATGGCTAGCATGGTGAGCAAAGGCG<br>
 <br>
-mkate ppr:<br>
+mkate ppr: GTCCCCCTGCAGGTTTATTAATTCAGTTTATG<br>
-mkate pprf:
+mkate pprf: TACATATGGCTAGCATGGTAAGCAAAGGTG<br>
 <br>
-frag4_rev:
+frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG<br>
-frag1_for:
+frag1_for: CGGCCGCTTCTAGAGACATTGATTATTTGCACGGCGT<br>
 <br>
-frag2_rev:
+frag2_rev: CAGTGTGATTATTAATTCAGTTTATGACCCAGGTTGCTCG<br>
-frag2_for:
+frag2_for: GGCTAGCGGAAAGAGGGGACAAATGTCAAAAATCC<br>
 <br>
-frag3_rev:
+frag3_rev: ATCAATGTAAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCT<br>
-frag3_for:
+frag3_for: TTAATAATCACACTGGCTCACCTTCGG<br>
 <br>
-frag4_rev:
+frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG<br>
-frag4_for:
+frag4_for: ATTATTTACATTGATTATTTGCACGGCGT<br>
 <br>
-frag5_rev:
+frag5_rev: CGGCCGCTACTAGTATTATCATTTGTACAGCTCGTCCATGC<br>
-frag5_for:
+frag5_for: TTTTTTTGGGCTAGCGGAAAGAGGGGACAAATGATTAACCG<br>
 <br>
-vector_rev:
+vector_rev: CTCTAGAAGCGGCCGCGA<br>
-vector_for:
+vector_for: TACTAGTAGCGGCCGCTGC<br>
 <br>
-Pre-PezT: GGA TCC TGC AGC GGC CGC TAC TAG TAC TCG AGC GCT GCT GCC A
+Pre-PezT: GGATCCTGCAGCGGCCGCTACTAGTACTCGAGCGCTGCTGCCA<br>
-For-PezT: ATA CCG AAT TCG CGG CCG CTT CTA GAT GGA AAT CCA AGA TTA TAC TG
+For-PezT: ATACCGAATTCGCGGCCGCTTCTAGATGGAAATCCAAGATTATACTG<br>
 <br>
 </body>
 </html>