"
Exeter/10 July 2013
From 2013.igem.org
(Difference between revisions)
Fentwistle (Talk | contribs) (→Liquid culture) |
Fentwistle (Talk | contribs) (→Afternoon) |
||
| Line 26: | Line 26: | ||
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | ||
| + | |||
| + | =(Next day, results of BBa_B0034 transformation)= | ||
| + | |||
| + | We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies. | ||
Revision as of 11:15, 16 July 2013
Morning
MiniPrep of the following Parts:
- BBa_K322122, cyan pigment
- BBa_K592012, magenta pigment
- BBa_K592010, yellow pigment
Plasmids extracted are now in storage at -20oC.
Afternoon
Liquid cultures of the blue light module and magenta pigment
- BBa_K592010, yellow pigment - BBa_K592012, magenta pigment - BBa_B0015, a terminator - BBa_K592005, FixJ protein intermediate - BBa_K592004, blue light sensor
Retry of BBa_B0034 transformation
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
(Next day, results of BBa_B0034 transformation)
We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.
