Team:Paris Bettencourt/YonatanTest
From 2013.igem.org
(Difference between revisions)
Line 30: | Line 30: | ||
.results { | .results { | ||
width:100%; | width:100%; | ||
- | height: | + | height:360px; |
margin-bottom:15px; | margin-bottom:15px; | ||
} | } | ||
.biocriks { | .biocriks { | ||
width:100%; | width:100%; | ||
- | height: | + | height:105px; |
} | } | ||
.mainfig { | .mainfig { |
Revision as of 09:07, 25 October 2013
![](https://static.igem.org/mediawiki/2013/d/d9/PB_achievementsbanenr.png)
Detect
Background
CRISPR/Cas systems generate site-specific double strand breaks and have recently been used for genome editing.
Aims
Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.
Results
- Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks.
- Testing the new assembly standard for our cloning.
![](https://static.igem.org/mediawiki/2013/1/1f/PB_phagesensor10.png)
After expression of the Cas9 and gRNA, the gRNA guides the Cas9 to the target sequence, the kanamycin resistance. There, the Cas9 generates a double strand break. This activates the SOS response. The reporter LacZ is under the pRECA promoter, which gets activated during the SOS response and we get hence a blue cell, if the resistance gene has successfully been detected.