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Team:DTU-Denmark/Notebook/6 July 2013
From 2013.igem.org
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=208 lab= | =208 lab= | ||
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== Main purposes today == | == Main purposes today == | ||
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Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants. | Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants. | ||
==Who was in the lab== | ==Who was in the lab== | ||
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Kristian, Helen | Kristian, Helen | ||
==Procedure== | ==Procedure== | ||
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Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html| link]). Used smaller amount though. | Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html| link]). Used smaller amount though. | ||
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== Conclusion from today == | == Conclusion from today == | ||
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The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP. | The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP. | ||
Revision as of 10:15, 26 July 2013
Contents |
208 lab
Main purposes today
Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.
Who was in the lab
Kristian, Helen
Procedure
Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html| link]). Used smaller amount though.
Then, viewed the periplasmic and cytoplasmic cell contents under UV light.
Conclusion from today
The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.
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