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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog
From 2013.igem.org
(Created page with "{{TeamBYUProvo}} <br> {| width="100%" | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Small Phage July - August Notebook: July 16 - July 31 Daily Log'''<...") |
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| - | <font size="4"> '''7/ | + | <font size="4"> '''7/17/13''' </font> |
| - | - | + | - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for [[Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation]] |
| - | - | + | - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage. |
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Revision as of 22:10, 17 July 2013
| Small Phage July - August Notebook: July 16 - July 31 Daily Log
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7/16/13 - Started approximately 60mL of BL21 liquid culture overnight.
7/17/13 - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for Team:BYU_Provo/Notebook/SmallPhage/Summer/Period1/Exp/7.13_Phage_Propagation - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
7/3/13 - Performed spot test for 6.27 Mutagen Concentration Test - Third Protocol to determine phage viability. 5.3 stock and phage from 6.12 Mutagen Concentration Test - Second Protocol were used as positive control. - Worked on the introduction for our outreach presentation.
7/7/13 - Started 60mL of BL21 liquid culture overnight.
7/8/13 - Made 750mL of x8 top agar. - We started our fourth round of mutagenesis in 7.8 Mutagen Concentration Test - Fourth Protocol
7/9/13 - Outreach @ Orem Public Library! - Started approximately 20mL of E coli BL21 liquid culture overnight.
7/10/13 - Performed titer to determine mutated phage concentration for 7.8 Mutagen Concentration Test - Fourth Protocol
7/11/13 - Started approximately 10mL of E coli BL21 liquid culture overnight.
7/12/13 - Performed spot test to confirm the titer result from July 10th. Detail procedure listed in 7.8 Mutagen Concentration Test - Fourth Protocol. - Started approximately 6 mL of E coli liquid culture overnight.
7/13/13 - Set up the phage liquid culture for propagation in 7.13 Phage Propagation Experiment.
7/14/13 - Started approximately 30mL of E coli BL21 liquid culture overnight.
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