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Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Dailylog
From 2013.igem.org
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7/16/13 - Started approximately 60mL of BL21 liquid culture overnight.
7/17/13 - The titer from 7.15 was too low to start an actual round of mutagenesis. One more round of propagation was performed for 7.13 Phage Propagation Experiment - Do we want to talk about this round of mutagenesis? phage stock 80e6. 1.5mL of E coli, 90uL of phage.
7/19/13 - Performed spot test for 7.17 Mutagen Concentration Test - Fifth Protocol - Performed titer to determine phage concentration 2 for 7.13 Phage Propagation Experiment
7/22/13 - A lot of ousekeeping:
7/26/13 - Designed protocols for 7.29 Mutagen Concentration Test - Sixth Protocol and decided to conduct an experiment to verify bacteria growth curve (specifics see 7.27 Bacteria Growth Curve After Dilution) - Started 3mL of BL21 liquid culture overnight.
7/27/13 - Conducted 7.27 Bacteria Growth Curve After Dilution
7/28/13 - Started 15mL of BL21 liquid culture overnight. - Started phage liquid culture to test for superinfection.
7/29/13 - Performed part 1 of 7.29 Mutagen Concentration Test - Sixth Protocol. - Did a spot test to determine the concentration phage in yesterday's liquid culture (We couldn't test for superinfection because all the bacteria were lysed.)
7/31/13 - Started 8mL BL21 liquid culture overnight and performed dilution series for the spot test procedure in 7.29 Mutagen Concentration Test - Sixth Protocol.
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