"
Exeter/10 July 2013
From 2013.igem.org
(Difference between revisions)
Fentwistle (Talk | contribs) (→Afternoon) |
Fentwistle (Talk | contribs) |
||
| Line 1: | Line 1: | ||
| - | |||
== Morning == | == Morning == | ||
| Line 13: | Line 12: | ||
| - | =Afternoon= | + | ==Afternoon== |
'''Liquid cultures of the blue light module and magenta pigment''' | '''Liquid cultures of the blue light module and magenta pigment''' | ||
| Line 31: | Line 30: | ||
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates! | ||
| - | =(Next day, results of BBa_B0034 transformation)= | + | ==(Next day, results of BBa_B0034 transformation)== |
We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies. | We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies. | ||
Revision as of 12:01, 18 July 2013
Morning
MiniPrep of the following Parts:
- BBa_K322122, cyan pigment
- BBa_K592012, magenta pigment
- BBa_K592010, yellow pigment
Plasmids extracted are now in storage at -20oC.
Afternoon
Liquid cultures of the blue light module and magenta pigment
- BBa_K592010, yellow pigment
- BBa_K592012, magenta pigment
- BBa_B0015, a terminator
- BBa_K592005, FixJ protein intermediate
- BBa_K592004, blue light sensor
Retry of BBa_B0034 transformation
Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!
(Next day, results of BBa_B0034 transformation)
We made two plates of BBa_B0034, each plate had 7 colonies. Our RFP control had 137 colonies.
